Histological Examination of the Osteogenic Properties of White Acropora Coral Grainules Sized 50–150 μm Under Subcutaneous Administration in the Experiment-Reference-Cited by-同舟云学术

Histological Examination of the Osteogenic Properties of White Acropora Coral Grainules Sized 50–150 μm Under Subcutaneous Administration in the Experiment

Published:2019-10-15 Issue:3 Volume:8 Page:72-78
ISSN:2225-7357
Container-title:Journal of Anatomy and Histopathology
language:
Short-container-title:Žurnal anatomii i gistopatologii

Author:

Suchkov D. I.¹,Pavlov A. V.¹

Affiliation:

1. I.P. Pavlov Ryazan State Medical University

Abstract

The aim of research was to study morphological characteristics of the osteogenic properties of the natural white Acropora coral skeleton granules when implanted under the skin.Material and methods. The study included 60 sexually mature male Wistar rats. The object of the morphological study was subcutaneous implants isolated for different periods of the experiment. The experimental animals were divided into three groups, 20 animals each. In the 1st and 2nd groups the granules were placed in a gelatin capsule, in the 3rd group – the granules were implanted directly under the skin of the animals. When preparing implants for the 1st group, the granules were treated with the blood of an animal taken from the tail vein during surgery. Implants for the 2nd group of animals were not treated with blood. Histological research methods (hematoxylin and eosin staining according to Mallory, Weigert–Van Gieson, and Masson–Goldner; staining to determine the age of fibrin (BioVitrum)) were used in the study; the results were statistically processed.Results. The results obtained demonstrated that heterotopic bone formation based on granules from the skeleton of natural coral was only possible when they were in wide contact with blood on the first day of the experiment. The results of the study also evidenced that blood clot elements, fibrocytes, and osteoblasts were the source for the development of newly formed bone tissues. The gelatin capsule allowed temporarily restricting coral granules from the surrounding tissues with peripheral venous blood cells inside; the fact contributing to the formation and accumulation of cellular elements and subsequently leading to the formation of young bone tissues.Conclusions. As demonstrated, subcutaneous implantation of coral granules in a gelatin capsule without blood did not result in osteogenesis. Due to the apparent osteoinductive potential, natural coral skeleton granules applied according to the proposed method are considered to be an alternative to other osteoplastic materials for the stimulation of de novo bone formation.

Publisher

VSMU N.N. Burdenko

Subject

General Earth and Planetary Sciences,General Environmental Science

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