THE ROLE OF THE INTESTINAL MICROBIOTA IN THE DEVELOPMENT OF INFLAMMATORY DISEASES OF THE INTESTINE

Abstract

Inflammatory bowel diseases (IBD) occupy a leading position in the structure of diseases of the gastrointestinal tract (GIT), as they are a progressive chronic pathology with an autoimmune type of inflammation. Changes in indicators of genus - species biodiversity can determine morphological changes at the latent stage of the disease. Target. Evaluation of the role of genus - species biodiversity of microbiota in patients with IBD. Materials and methods. Performed 16s-rRNA sequencing of the gut microbiota in 15 patients with confirmed ulcerative colitis (UC) and 3 patients with Crohn's disease and 20 healthy controls. Also, an analysis of the parameters of the general blood test (CBC), biochemical blood test (C - reactive protein (CRP)) was carried out. Statistical data processing was carried out using Statistics programs. Results. Among the studied laboratory parameters in patients with IBD, there was a significant increase in CRP - 19.61.5 mg/l, leukocytosis - 11.40.68 g/l, including neutrophilosis - 742,1% according to compared with the control group. As a result of sequencing of the intestinal microbiota, there was a decrease in the normobiota, as well as an increase in the representatives of the pathogenic cluster. Conclusion. A decrease in the biodiversity of the microbiota in patients with IBD compared with the control group, a significant change in the pathogenic cluster, an imbalance in the representatives of Bacteroidetes and Firmicutes were revealed, which confirms the role of the intestinal microbiota in the development of IBD.