Investigation of the effect of Coenzyme–Q10 on Cyclophosphamide induced testicular damage in male rats

Author:

Koşal Volkan1ORCID,Rua İhsan2ORCID,Yüksek Veysel3ORCID,Keleş Ömer Faruk4ORCID

Affiliation:

1. Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Artificial Insemination. Van, Turkey

2. Private Clinic, Department of Obstetrics and Gynecology. Van, Turkey

3. Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Biochemistry. Van, Turkey

4. Van Yuzuncu Yil University, Faculty of Veterinary Medicine, Department of Pathology. Van, Turkey

Abstract

Cyclophosphamide (CP) is one of the frequently preferred chemotherapeutic agents Worldwide. CP has negative effects on the testes, spermatogenesis, and reproductive hormones. The aim of this study was to determine the protective effect of Coenzyme Q10 (CoQ10) on the damage caused by CP. CoQ10 is use in the treatment of infertility problems and is naturally found in the testes and seminal fluid. Thirty–six Albino Wistar male rats were divided into six groups (Control, Sham, Cyclophosphamide (CP), Coenzyme Q10 (CoQ10), CP + CoQ10 I, CP + CoQ10 II), with six animals in each group. Semen analysis included investigations of sperm DNA damage, motility, abnormal sperm ratio, and density. Histopathological examination and assessment of oxidative stress parameters in the testes were conducted. Additionally, serum levels of FSH, LH, and Testosterone were measured. CoQ10 administration increased the motility rate, density, and Testosterone levels in testicular damage caused by CP (P<0.05). Furthermore, it was observed that the abnormal sperm ratio, sperm DNA damage, and oxidative stress were reduced (P<0.05). Based on the results of this study, the use of CoQ10 in conjunction with CP has the potential to alleviate male infertility problems that may arise from CP administration.

Publisher

Universidad del Zulia

Subject

General Veterinary

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