Interaction of herpesviruses with mature human spermatozoa in the model system <i>in vitro</i>-Reference-Cited by-同舟云学术

Interaction of herpesviruses with mature human spermatozoa in the model system <i>in vitro</i>

Published:2016-06-28 Issue:3 Volume:61 Page:119-125
ISSN:2411-2097
Container-title:Problems of Virology
language:
Short-container-title:Problems of Virology

Author:

Malolina E. A.^ORCID,Lebedeva A. L.,Kulibin A. Yu.^ORCID,Evdokimov V. V.^ORCID,Kurilo L. F.^ORCID,Sorokina T. M.,Tulenev Ju. A.,Naumenko V. A.,Kushch A. A.^ORCID

Abstract

The DNA of human herpesviruses (HHV), including the herpes simplex virus (HSV) and cytomegalovirus (CMV), is often identified in ejaculates of patients with urogenital diseases and infertility. At least a part of viral DNA is associated with cell fraction of ejaculate. However, it remains unclear how the semen is infected by the virus. It can be located in gametes or be capable of infecting mature germ cells, including motile sperm cells. In order to resolve this issue, interactions of the CMV and HSV with human sperm cells were studied using an original optimized model of the herpesviral infection of male gametes in vitro. The analysis of the immunofluorescent staining of gametes for viral antigens has shown that CMV infected 2% gametes, while HSV infected 17.26 ± 2.58% gametes. The fraction of progressively motile sperm cells contained 13.99 ± 4.64% infected cells. Localization of HSV was studied by the confocal microscopy. Sometimes, viral gB protein was found on sperm cell membrane. In addition, optical scanning of other cells has shown the intracellular localization of the viral proteins. In the majority of spermatozoa, the viral proteins were observed in the head and neck. In some cells, they were located in the middle piece or, rarely, in the equatorial segment. In general, after in vitro infection HSV antigens were located in the same areas of the sperm cells as in ejaculates from infected patients. According to DNA–DNA hybridization in situ, gametes containing HSV DNA accounted for 16.94 ± 5.28%, which is consistent with the results obtained in the immunofluorescence assay. It can be concluded that mature male gametes are infected by HHV in the genital tract, where the virus binds to the sperm cell membrane and enters the cell. Interaction of HHV with progressively motile sperm cells implies a vertical viral transmission upon fertilization and points to the necessity of testing ejaculate for herpesviruses infections.

Publisher

Central Research Institute for Epidemiology

Subject

Infectious Diseases,Virology,General Medicine

Reference23 articles.

1. Dejucq N., Jégou B. Viruses in the mammalian male genital tract and their effects on the reproductive system. Microbiol. Mol. Biol. Rev. 2001;65(2): 208–31.

2. Kaspersen M.D., Höllsberg P. Seminal shedding of human herpesviruses. Virol. J. 2013; 10: 226.

3. Kapranos N., Petrakou E., Anastasiadou C., Kotronias D. Detection of herpes simplex virus, cytomegalovirus, and Epstein–Barr virus in the semen of men attending an infertility clinic. Fertil. Steril. 2003; 79(3): 1566–70.

4. Michou V., Liarmakopoulou S., Thomas D., Tsimaratou K., Makarounis K., Constantoulakis P. et al. Herpes virus infected spermato-following density gradient centrifugation for IVF purposes. Andrologia. 2012; 44(3): 174–80.

5. Chen M., Cai L.Y., Kanno N., Kato T., Lu J., Jin F. et al. Detection of human herpesviruses (HHVs) in semen of human male infertile patients. J. Reprod. Dev. 2013; 59(5): 457–62.