Author:
Iunikhina O. V.,Kompanets G. G.
Abstract
Survival of viruses in the environment is a very important problem in epidemiology, especially for infections with indirect transmission. This work describes the results of the experimental study of adsorption and survival of the hantavirus on different environmental substrates (natural organic and inorganic sorbents). Bovine serum albumin (BSA) solution (5-10%) was effective in the hantavirus elution and phosphate-buffer saline (PBS) pH 7,2 was optimal for elution of specific RNA. Potential survival of the infectious hantavirus on environmental substrates was observed within up to 14 days at +4°С.
Publisher
Central Research Institute for Epidemiology
Subject
Infectious Diseases,Virology,General Medicine
Reference12 articles.
1. Schmaljohn C., Hjelle B. Hantaviruses: a global disease problem. Emerg. Infect. Dis. 1997; 3 (2): 95–104.
2. Hardestam J., Simon M., Hedlund K.O., Vaheri A., Klingstrom J., Lundkvist A. Ex vivo stability of the rodent-borne Hantaan virus in comparison to that of arthropod-borne members of the Bunyaviridae family. Appl. Environ. Microbiol. 2007; 73 (8): 2547–51.
3. Kallio E.R., Klingstrom J., Gustafsson E., Manni T., Vaheri A., Henttonen H. et al. Prolonged survival of Puumala hantavirus outside the host: evidence for indirect transmission via the environment. J. Gen.Virol. 2006; 87 (8): 2127–34.
4. Иунихина О.В., Компанец Г.Г., Слонова Р.А. Способность хантавируса адсорбироваться на почвообразующих минеральных частицах. Дальневосточный журнал инфекционной патологии. 2008; 13: 134–8.
5. Lee P.W., Gibbs C.J., Gajdusek D.C., Yanagihara R.T. Serotypic classification of hantavirus by indirect immunofluorescent antibody and plaque reduction neutralization tests. J. Clin. Microbiol. 1985; 22 (6): 940–4.