LEVELS OF H. PYLORI ANTIBODIES AND INFLAMMATORY MARKERS IN BLOOD SERUM SAMPLES OF MOSCOW RESIDENTS

Abstract

Introduction. Helicobacter pylori (Hр) is considered usually as one of the factors in the development of gastritis, peptic ulcer, and gastric cancer, and at the same time as protection from asthma, diabetes type II, and obesity. This study was carried out with a goal to evaluate possible linkage between the levels of circulating anti-Hp antibodies and inflammatory markers - IL-6, IL-8 and malonic dialdehyde (MDA) - in cross-sectional study of Moscow adults. Material and Methods. Serum samples of Moscow working-aged residents (both gender, N=252) were used for evaluation of antibodies to lysate Hp antigen/recombinant CagA and cytokines IL-6, IL-8 with corresponding ELISA kits. MDA was determined by reaction with thiobarbituric acid. Results. Standard correlation analysis didn’t reveal any significant association between the levels of circulating antibodies against the applied commercial antigens and the levels of IL-6, IL-8 and MDA. Nevertheless, two parallel branches of the experimental points in seropositive area of anti-CagA/IL-8 scatterplot (but not for IL-6 and MDA variables) were clearly seen and correspond to two maximums at bimodal anti-CagA distribution. The data obtained are consistent with typical for CagA outstrip in IL-8 induction and testify the existence of two human genotypes having ~2-fold difference in antibody response but the same reactivity of CagA - IL-8 axis. Since CagA-positive IL-8 levels are of one order with CagA-negative ones (from another organs) and signs of concomitant oxidative stress were not revealed, the data obtained can be interpreted equally as early pathologic or actual symbiotic events. Conclusion. Quantitative assessment of circulating anti-Hp antibodies, together with more detail mathematical analysis, will increase contributions of population studies to investigation of equilibrium between Hp and human organisms.