EVALUATION OF BONE TISSUE REGENERATION USING A COMBINATION OF AUTOLOGOUS FIBRIN MATRIX WITH PRE-DIFFERENTIATED MESENCHYMAL STEM CELLS IN AN EXPERIMENTAL MODEL

Abstract

Introduction. According to domestic and foreign authors, the frequency of damage to the mandible bone is 36-84% of all damage to the bones of the facial skeleton. Since A.Y. Friedenstein made a key contribution to the study of mesenchymal stem cells (MSC), their analysis, types, methods of isolation and use in various combinations with materials, allowed to expand the boundaries of regenerative medicine. Aim of the study. The purpose of the study is to evaluate the regenerative potential of cortical bone tissue in the region of the mandible angle, with the introduction of exogenous pre-differentiated MSC (MSCs) on the autologous fibrin matrix in the experiment. Material and methods. We carried out an experimental study on 27 rabbits of the Soviet Chinchilla breed. Animals were divided into 3 groups: A, B and control. The bone defect diameter of 6mm was formed in the angle of the mandible of all animals with a trepan. Groups A and B mandible defects were injected with autologous fibrin clot with pre-differentiated MSCs in the amount of 300 thousand and 1 million cells, respectively. Defect size was assessed after 8, 10 and 12 weeks. Results. At week 12 after surgery, in the experimental group B the size of the bone tissue defect was significantly reduced and amounted to 0.006022 ± 0.00135 mm3, while in the control group the defect volume was 0.05597 ± 0.00505 mm3, and in group A was 0.02235 ± 0.0056 mm3. In an experimental study, an improvement was obtained in the pathophysiological processes of bone tissue regeneration under conditions of a bicortical defect with minimal presence of spongy substance in the damage zone.