-455A allele of FGB gene in differential diagnostics of acute infective endocarditide and sepsis.

Abstract

Formation of infective endocarditis (IE), i.e. septic thrombosis of the endocardium may depend on the genetically determined thrombophilia. The aim of this work was the search for the genetic markers of IE in the hemostatic system. 67 patients (20 women and 47 men) with IE, including 35 men and 12 women with acute and 12 men and 8 women with subacute forms of the disease, and 61 patients (28 women и 33 men) with sepsis were genotyped on polymorphic loci -455G-A in the beta-fibrinogen gene (FGB), Leu33Pro in the integrin beta-3 gene (ITGB3), Ala222Val (C677T) in the methylenetetrahydrofolate reductase gene (MTHFR) by allele-specific polymerase chain reaction. The association of single nucleotide polymorphisms, -455G-A of FGB gene and Ala222Val of MTHFR gene, with the risk of development of acute IE was revealed in comparison with sepsis. The greatest (11-fold) risk was found in male patients of different ages bearing the genotype -455A-A of the β-fibrinogen FGB gene. Thus, a susceptibility to thrombus formation, dependent on hemostasis genes, can promote the development of acute infective endocarditis.