ANTIMICROBIAL RESISTANCE AND CLINICAL SIGNIFICANT RESISTANCE MECHANISMS OF SALMONELLA ISOLATED IN 2014-2018 IN ST.PETERSBURG, RUSSIA

Author:

Egorova Svetlana A.1ORCID,Kaftyreva Lidiya A.2ORCID,Suzhaeva Lyudmila V.1ORCID,Zabrovskaia Anna V.1ORCID,Voitenkova Elena V.1ORCID,Matveeva Zoya N.1ORCID,Ostankova Yulia V.1ORCID,Likhachev I. V.1,Satosova N. V.3,Kitsbabashvili R. V.4,Smirnova E. V.5,Semchenkova L. I.5,Bystraya T. E.5,Sokol’nik S. E.6,Utkina N. P.6,Sikhando L. Y.6

Affiliation:

1. Saint-Petersburg Pasteur Institute

2. Saint-Petersburg Pasteur Institute; State Educational Institution of the Higher Professional Education «North-Western state medical University n.a. I.I. Mechnikov» of the Ministry of Health of the Russian Federation

3. State Educational Institution of the Higher Professional Education «North-Western state medical University n.a. I.I. Mechnikov» of the Ministry of Health of the Russian Federation

4. ANO «Medical Center XXI Century»

5. FBUZ «Center for Hygiene and Epidemiology in St. Petersburg», division № 3

6. FBUZ «Center for Hygiene and Epidemiology in St. Petersburg», division № 6

Abstract

The article presents the results of antimicrobial resistance monitoring of Salmonella isolated from children and adults with diarrhea in St. Petersburg in 2014-2018. In 746 isolates of 42 serovars more than 90,0% belonged to three: S. enteritidis (79,6%), S. typhimurium (6,8%) and S. infantis (3,8%). The antimicrobial susceptibility testing (according the EUCAST) to 7 classes of antimicrobials revealed the resistance in 78,6% of Salmonella. Low-level quinolone resistance (MIC of ciprofloxacin 0,12-0,5 mg/l) was detected in 63,3% isolates (S. enteritidis -71,0%, S. typhimurium - 15,7%, S. infantis - 89,3%) and was due to five kinds of single nucleotide substitutions in gyrA: Asp87Tyr - 36,1% of studied isolates (only S. infantis); Ser83Phe - 22,2% (only S. enteritidis); Asp87Asn - 19,4% (S. enteritidis, S. typhimurium, S. hadar, S. newport); Ser83Tyr -11,1% (S. enteritidis and S. infantis) and Asp87Gly - 8,3% (only S. enteritidis). Only in one S. kentucky isolate with high-level fluoroquinolone resistance (MIC of ciprofloxacin > 8,0 mg/l) two substitutions (Ser83Phe and Asp87Asn) were detected. Two Salmonella isolates (S. typhimurium and S. corvallis) had plasmid-mediated quinolone resistance (qnrS). Extended-spectrum cephalosporin resistance was found in 6 Salmonella serovars (1,6%). The bla-genes were detected: of genetic group CTX-M1 - in 10 isolates (serovars S. typhimurium, S. enteritidis, S. abony, S. coeln and S. virchow), CTX-M2 - in 2 S. typhimurium isolates, CTX-M9 - in three S. enteritidis isolates. In one S. typhimurium CTX-M1 and CTX-M2 were detected. The gene of CMY-2 (molecular class C cephalosporinase) was revealed in two isolates (S. newport and S. enteritidis). Our study showed that Salmonella (the main bacterial pathogen of acute diarrhea in children and adults) isolated in Saint-Petersburg had antimicrobial resistance to drugs of choice for salmonellosis treatment.

Publisher

EKOlab

Subject

Biochemistry, medical,Medical Laboratory Technology,General Medicine

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