Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy

Author:

Kitamura Akira1,Kinjo Masataka1

Affiliation:

1. Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University

Publisher

Biophysical Society of Japan

Subject

General Medicine

Reference8 articles.

1. [1] Mikuni, S., Tamura, M. & Kinjo, M. Analysis of intranuclear binding process of glucocorticoid receptor using fluorescence correlation spectroscopy. FEBS Lett. 581, 389–393 (2007).

2. [3] Aoki, K., Kumagai, Y., Sakurai, A., Komatsu, N., Fujita, Y., Shionyu, C., et al. Stochastic ERK activation induced by noise and cell-to-cell propagation regulates cell density-dependent proliferation, Mol. Cell 52, 529–540 (2013).

3. [5] Matsuda, T. & Nagai, T. Quantitative measurement of intracellular protein dynamics using photobleaching or photoactivation of fluorescent proteins. Microscopy (Oxf) 63, 403–408 (2014).

4. [10] Kitamura, A., Nakayama, Y. & Kinjo, M. Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding. Biochem. Biophys. Res. Commun. 463, 401–406 (2015).

5. [11] Kitamura, A., Nakayama, Y., Shibasaki, A., Taki, A., Yuno, S., Takeda, K., et al. Interaction of RNA with a C-terminal fragment of the amyotrophic lateral sclerosis-associated TDP43 reduces cytotoxicity. Sci. Rep. 6, 19230 (2016).

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