Bottom-up creation of cell-free molecular systems: Basic research toward social implementation
Author:
Affiliation:
1. Graduate School of Engineering Science, Yokohama National University
2. Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology (TUAT)
3. Earth-Life Science Institute, Tokyo Institute of Technology
Publisher
Biophysical Society of Japan
Subject
Physiology,Molecular Biology,Biophysics,Biochemistry,Biochemistry, Genetics and Molecular Biology (miscellaneous)
Link
https://www.jstage.jst.go.jp/article/biophysico/20/4/20_e200042/_pdf
Reference15 articles.
1. [1] Shimizu, Y., Inoue, A., Tomari, Y., Suzuki, T., Yokogawa, T., Nishikawa, K., et al. Cell-free translation reconstituted with purified components. Nat. Biotechnol. 19, 751–755 (2001). https://doi.org/10.1038/90802
2. [2] Shimojo, M., Amikura, K., Masuda, K., Kanamori, T., Ueda, T., Shimizu, Y. In vitro reconstitution of functional small ribosomal subunit assembly for comprehensive analysis of ribosomal elements in E. coli. Commun. Biol. 3, 142 (2020). https://doi.org/10.1038/s42003-020-0874-8
3. [3] Hibi, K., Amikura, K., Sugiura, N., Masuda, K., Ohno, S., Yokogawa, T., et al. Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs. Commun. Biol. 3, 350 (2020). https://doi.org/10.1038/s42003-020-1074-2
4. [4] Ogawa, M., Uyeda, A., Harada, K., Sato, Y., Kato, Y., Watanabe, H., et al. Class III polyphosphate kinase 2 enzymes catalyze the pyrophosphorylation of adenosine-5'-monophosphate. ChemBioChem 20, 2961–2967 (2019). https://doi.org/10.1002/cbic.201900303
5. [5] Fujii, S., Matsuura, T., Sunami, T., Nishikawa, T., Kazuta, Y., Yomo, T. Liposome display for in vitro selection and evolution of membrane proteins. Nat. Protocol. 9, 1578–1591 (2014). https://doi.org/10.1038/nprot.2014.107
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