PREVALENCE AND CHARACTERISATION OF EXTENDED-SPECTRUM BETA-LACTAMASES AND PLASMID-MEDIATED QUINOLONES RESISTANCE IN Enterobacteriaceae ISOLATED FROM COMPANION ANIMALS-Reference-Cited by-同舟云学术

PREVALENCE AND CHARACTERISATION OF EXTENDED-SPECTRUM BETA-LACTAMASES AND PLASMID-MEDIATED QUINOLONES RESISTANCE IN Enterobacteriaceae ISOLATED FROM COMPANION ANIMALS

Published:2024-01-15 Issue:4(196)/2023 Volume:56 Page:541-549
ISSN:2784-0360
Container-title:Journal of Applied Life Sciences and Environment
language:en
Short-container-title:JALSE

Author:

COZMA Andreea Paula¹^ORCID,MACIUCA Iulia Elena²^ORCID,RIMBU Cristina Mihaela³^ORCID,MOROSAN Serban⁴^ORCID,TRINCA Lucia Carmen⁵^ORCID,TIMOFTE Dorina⁶^ORCID

Affiliation:

1. 1Department of Exact Sciences, Faculty of Horticulture, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 3, Mihail Sadoveanu Alley, 700490, Iasi, Romania

2. Department of Veterinary Anatomy, Physiology and Pathology, School of Veterinary Science, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom

3. Department of Public Health, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 8, Mihail Sadoveanu Alley, 700489, Iasi, Romania

4. Department of Public Health, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 8, Mihail Sadoveanu Alley, 700489, Iasi, Romania; Sorbonne University, UMS 28 INSERM, Paris, France

5. Department of Exact Sciences, Faculty of Horticulture, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 3, Mihail Sadoveanu Alley, 700490, Iasi, Romania

6. Department of Public Health, Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 8, Mihail Sadoveanu Alley, 700489, Iasi, Romania; Department of Veterinary Anatomy, Physiology and Pathology, School of Veterinary Science, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Neston, United Kingdom

Abstract

Antimicrobial resistance is a major public health concern worldwide. This study aims to determine the prevalence of Enterobacterales producing beta-lactamase (TEM, SHV, OXA) or extended-spectrum beta-lactamases (ESBL), as well as plasmid-mediated resistance to quinolones (PMQR) (qnrA, qnrB, qnrS) in companion animals from the northeast region of Romania. A total of 124 faecal samples were collected aseptically from healthy dogs attending the veterinary practice for vaccination and cultivated on Brilliance ESBL medium (Oxoid, UK). The ESBL production testing was performed using the combination disc test. The identification of Enterobacterales strains was achieved using molecular identification and based on biochemical tests. Antimicrobial susceptibility testing was performed using the disk diffusion method. Identification of genes encoding for beta-lactamase enzymes and genes encoding plasmid-mediated resistance to quinolones was performed by PCR according to the protocols previously described. After ESBL screening, 31 (31/124; 25%) extended-spectrum cephalosporin (ESC)-resistant Enterobacterales were obtained, and 67.74% (21/31) of them were confirmed as ESBL-producers. Regarding the Enterobacterales species, 27 (27/31; 87.1%) were Escherichia coli and 4 (4/31; 12.9%) strains were Klebsiella pneumoniae. Among the ESBL-producing isolates, the blaCTX-M-1 gene group was predominant (58.82%), followed by the blaCTX-M-9 group (41.18%). The blaTEM, blaSHV and blaOXA gene groups were identified in 54.83%, 29.03% and 3.22% of the analysed strains, respectively. The prevalence of PMQR genes was 22.58% and consisted only of qnrS (19.35%) and qnrA (3.22%) genes. The prevalence of ESBL strains related to the total number of analysed samples was 16.93% (21/124). The findings show a significant prevalence of ESBLs and PMQR genes in Enterobacterales strains isolated from the faeces of healthy dogs, implying that pets may pose a risk of transmitting ESBL strains to other animals or owners.

Publisher

University of Life Sciences in lasi

Subject

General Medicine

Link

https://jurnalalse.com/wp-content/uploads/ALSE4-2023-05.pdf

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