THE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY

Author:

TANG ZHI-LIE ,LIANG RUI-SHENG ,CHANG HONG-SEN ,

Abstract

The Imaging theory of two-photon and multi-photon confocal scanning microscopy is presented. The three-dimensional point spread function and three-dimensional optical transfer function of two-photon confocal scanning microscopy is derived. By using the three-dimensional point spread function and three-dimensional optical transfer function theory, we have proved that the horizontal and vertical resolutions of two-photon confocal scanning microscopy are higher than those of the single photon confocal scanning microscopy, and the space resolution of multi-photon confocal scanning microscopy is higher than that of two-photon confocal scanning microscopy.

Publisher

Acta Physica Sinica, Chinese Physical Society and Institute of Physics, Chinese Academy of Sciences

Subject

General Physics and Astronomy

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