Affiliation:
1. samarra
2. Al-Salam University College
3. University of Kirkuk
Abstract
The aim of this study is isolate and identify fungi from different habitat in Kirkuk City - Iraq. The fungal species were isolated from soil and water in four season 2021-2022, collected the samples from various geographical habitat in Kirkuk City. The fungi isolation from soil and water done by inoculating (1ml) from serial dilutions on Potato Dextrose Agar (PDA) plates. The molecular identification of the isolated fungi at the species level, by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in GenBank (NCBI). Seven fungal species were identified. The results showed that the (Aspergillus flavus 20.83%) was the most abundant fungus, while the (Penicillium citrinum 8.30%) was the less prevalent one in all resources and locations. The seven local fungal isolates were registered within NCBI, and this is the first record of these isolates in Iraq
Reference14 articles.
1. Anderson IC, Parkin PI. 2007. Detection of active soil fungi by RT-PCR amplification of precursor rRNA molecules. Microbiol Methods. 68:248–253.
2. Bermingham S, Maltby L, Cooke RC. 1996. Effect of acoal mine effluent on aquatic hyphomycetes. Field Study Appl Ecol. 33: 1311-1321.
3. Cappccino JG, Sherman N. 1996. Microbiology. alaboratory manual. 4th edu. Benjamin. Cummings pub.
4. Chandrashekar MA, Soumya Pai K, Raju NS. 2014. Fungal Diversity of Rhizosphere Soils in Different Agricultural fields of Nanjangud Taluk of Mysore District. Karnataka. India. Int.J.Curr.Microbiol.App.Sci. 3:559-566.
5. Gaddeyya G, Niharika PS, Bharathi P, Kumar PKR. 2012. Isolation and identification of soil mycoflora in different crop fields at Salur Mandal. AdvAppl Sci Res. 3:2020-2026.