Sugar beet cells cellular and extracellular events taking place in response to drought and salinity
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Published:2023-05-19
Issue:2
Volume:82
Page:128-141
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ISSN:1847-8476
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Container-title:Acta botanica Croatica
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language:
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Short-container-title:Acta bot. Croat. (Online)
Author:
Pavoković Dubravko1, Horvatić Anita2, Tomljanović Ingrid3, Balen Biljana1, Krsnik-Rasol Marijana1
Affiliation:
1. University of Zagreb, Faculty of Science, Department of Biology, Horvatovac 102a, 10000 Zagreb, Croatia 2. University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Chemistry and Biochemistry, 10000 Zagreb, Croatia 3. GenomeScan B.V., Plesmanlaan 1D, 2333 BZ Leiden, Netherlands
Abstract
Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.
Publisher
University of Zagreb, Faculty of Science, Department of Biology
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics
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