In vitro cultivation and biocontrol potential of Botryosphaeria visci against European mistletoe (Viscum album L.)
-
Published:2023-05-31
Issue:2
Volume:82
Page:109-116
-
ISSN:1847-8476
-
Container-title:Acta botanica Croatica
-
language:
-
Short-container-title:Acta bot. Croat. (Online)
Author:
Bilonozhko Yuliia1ORCID, Krupodorova Tetiana1, Rabokon Anastasiia1, Postovoitova Anastasiia1, Kalafat Lubov1, Pirko Yaroslav1, Blume Yaroslav1
Affiliation:
1. Institute of Food Biotechnology and Genomics of Natl. Academy of Sciences of Ukraine, Baidy-Vyshnevetskogo Str 2а, 04123 Kyiv, 123, Ukraine
Abstract
To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.
Publisher
University of Zagreb, Faculty of Science, Department of Biology
Subject
Plant Science,Ecology, Evolution, Behavior and Systematics
Reference33 articles.
1. Varga, I., Nagy, V., Baltazár, T., Mátyás, K.K., Poczai, P., Molnár, I., 2012b: Különbözô szisztémikus herbicidek fehér fagyöngy (Viscum album) elleni hatékonyságának, illetve a fagyöngy hiperparazita kórokozójára gyakorolt antifungisztatikus hatásának vizsgálata. Növényvédelem 48, 507–517. (Study of the efficiency of different systemic herbicides against European mistletoe (Viscum album) and their antifungal activity against hyperparasitic mistletoe fungus). 2. Sanguesa-Barreda, G., Linares, J.C., Camarero, J.J., 2013: Drought and mistletoe reduce growth and water-use efficiency of Scots pine. Forest Ecology and Management 296, 64–73.http://dx.doi.org/10.1016/j.foreco.2013.01.028. 3. Sutton, B.C., 1980: The Coelomycetes. Fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute (CMI). Kew, Surrey, England. 4. Thomas, P.A., Dering, M., Giertych, M.J., Iszkuło, G., Tomaszewski, D., Briggs, J., 2022: Biological Flora of Britain and Ireland: Viscum album. Journal of Ecology303, 1–39.http://dx.doi.org/10.1111/1365-2745.14036. 5. Tkaczyk, M., Sikora, K., 2020: First report about occurance of Sphaeropsis visci on mistletoe (Viscum album L.) in Poland. Baltic Forestry 26, 2–4. http://dx.doi.org/10.46490/BF461.
|
|