Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC-Reference-Cited by-同舟云学术

Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

Published:2019-03-18 Issue:1 Volume:25 Page:65-69
ISSN:1735-403X
Container-title:Pharmaceutical Sciences
language:en
Short-container-title:Pharm Sci

Author:

Bhat Musadiq Hussain¹^ORCID,Fayaz Mufida¹,Kumar Amit²,Dar Alamgir Ahmad³,Jain Ashok Kumar²

Affiliation:

1. School of Studies in Botany, Jiwaji University, Gwalior-474011, India.

2. Institute of Ethnobiology, Jiwaji University, Gwalior-474011, India.

3. Research Centre for Residue and Quality Analysis, Sher‐e‐Kashmir University of Agricultural Sciences and Technology (SKUAST‐K), Srinagar, J&K- 180009, India.

Abstract

Background: The present study was carried out for determination of amino acid content in tubers of Dioscorea bulbifera using reverse-phase high-performance liquid chromatography. Methods: The method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of AccQ-Fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (HPLC-PDA) at 254 nm having column temperature of 37 ºC. Results: The proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. Methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. Conclusion: The method is reliable, simple and economical for determining the amino acid content of Dioscorea bulbifera tubers.

Publisher

Maad Rayan Publishing Company

Subject

General Pharmacology, Toxicology and Pharmaceutics,Pharmaceutical Science

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