Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC-Reference-Cited by-同舟云学术

Chromatographic Method for Determination of the Amino Acid Content in Dioscorea bulbifera L. Tubers by RP-HPLC

Published:2019-03-18 Issue:1 Volume:25 Page:65-69
ISSN:1735-403X
Container-title:Pharmaceutical Sciences
language:en
Short-container-title:Pharm Sci

Author:

Bhat Musadiq Hussain¹^ORCID,Fayaz Mufida¹,Kumar Amit²,Dar Alamgir Ahmad³,Jain Ashok Kumar²

Affiliation:

1. School of Studies in Botany, Jiwaji University, Gwalior-474011, India.

2. Institute of Ethnobiology, Jiwaji University, Gwalior-474011, India.

3. Research Centre for Residue and Quality Analysis, Sher‐e‐Kashmir University of Agricultural Sciences and Technology (SKUAST‐K), Srinagar, J&K- 180009, India.

Abstract

Background: The present study was carried out for determination of amino acid content in tubers of Dioscorea bulbifera using reverse-phase high-performance liquid chromatography. Methods: The method involved the vapor phase hydrolysis of the sample, automated derivatisation of the amino acids with the aid of AccQ-Fluor reagent kit, separated on a high performance liquid chromatography equipped with photo diode array (HPLC-PDA) at 254 nm having column temperature of 37 ºC. Results: The proportional molar concentration for each amino acid was calculated based on the concentration of standard amino acids and expressed as μg amino acid/mg sample. Methionine, aspartic acid and leucine were major components while as tyrosine was found minor from the plant on dry weight basis. Conclusion: The method is reliable, simple and economical for determining the amino acid content of Dioscorea bulbifera tubers.

Publisher

Maad Rayan Publishing Company

Subject

General Pharmacology, Toxicology and Pharmaceutics,Pharmaceutical Science

Reference21 articles.

1. Antitumor-Promoting Constituents from Dioscorea bulbifera L. in JB6 Mouse Epidermal Cells.

2. Antidiabetic Activity ofGnidia glaucaandDioscorea bulbifera: Potent Amylase and Glucosidase Inhibitors

3. Comparative Nutritional and Phytochemical Evaluation of the Aerial and Underground Tubers of Air Potato (Dioscorea bulbifera) Available in Abakaliki, Ebonyi State, Nigeria

4. Determination of 21 free amino acids in beer by HPLC with AQC pre-column derivatization;Li M;Se Pu,2007

5. Amino acid determination in grape juices and wines by HPLC using a modification of the 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) method;Hernández-Orte P;Chromatographia,2003

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