Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan-Reference-Cited by-同舟云学术

Prevalence of Occult Hepatitis B Virus Infections Among Kidney Transplant Patients in Khartoum State, Sudan

Published:2020-01-15 Issue: Volume: Page:80-84
ISSN:2394-4099
Container-title:International Journal of Scientific Research in Science, Engineering and Technology
language:en
Short-container-title:IJSRSET

Author:

Mustafa Mashaer M¹,El Hussein Abdel Rahim M²,Elkhidir Isam M³,A Enan Khalid²

Affiliation:

1. Department of Medical microbiology, Faculty of Medical Laboratory Science, El Neelain University, Khartoum, Sudan

2. Department of Virology, Central Laboratory- The Ministry of Higher Education and Scientific Research, P.O. Box 7099, Khartoum, Sudan

3. Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan

Abstract

This study was carried out to detect occult hepatitis B virus (OHB) among renal transplant patients in Khartoum State, Sudan. Antigen capture enzyme linked immunosorbent assay (ELISA) competitive ELISA and polymerase chain reaction (PCR) were used to respectively detect hepatitis B surface antigen (HBsAg), ; Hepatitis B core antibody (HBcAb) and hepatitis B virus (HBV) DNA in 100 plasma samples collected from patients during the period from May to October 2018 . Out of the 100 patients sampled, 70 were males and 30 were females (age 15 to 65 years) and none of these patients showed signs of clinical hepatitis. The results showed that 3 out of the 100 samples were positive for HBsAg, and were subsequently excluded from the study. Out of the remaining HBsAg negative 97 samples, 39(40.2%) showed positive HBcAb and none (0%) tested positive to HBV DNA using competitive ELISA and PCR, respectively. These results indicated that more investigations including more patients from other transplant centers are needed to fully elucidate the situation of occult hepatitis B in renal transplant patients in Sudan.

Publisher

Technoscience Academy

Subject

General Medicine

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