Affiliation:
1. Amity University Kolkata, D. Y. Patil School of Biotechnology and Bioinformatics, Sri Venkateswara College of Engineering, Yuvaraja's College (Autonomous), Chennai, Tamil Nadu, India
Abstract
The present review is based on papers published between 1990 and 2020 and gives Comparative information about the most common protein purification techniques Gel-Filtration, Chromatography, Ion-Exchange Chromatography, Electrophoresis, Affinity Chromatography, and Dialysis, High-Pressure Liquid Chromatography. and their applications.
Reference44 articles.
1. Tan, Z. jian, Li, F. fang, Xu, X. lei & Xing, J. min. Simultaneous extraction and purification of aloe polysaccharides and proteins using ionic liquid based aqueous two-phase system coupled with dialysis membrane. Desalination 286, 389–393 (2012).
2. Palanirajan, S. K., Govindasamy, P. & Gummadi, S. N. Polystyrene adsorbents: rapid and efficient surrogate for dialysis in membrane protein purification. Sci. Rep. 10, 1–9 (2020).
3. Gelunaite, L., Lukša, V., Sud?iuviene, O., Bumelis, V. & Pesliakas, H. Chelated mercury as a ligand in immobilized metal ion affinity chromatography of proteins. J. Chromatogr. A 904, 131–143 (2000).
4. GK, M. When to Avoid Dialysis during Protein Purification? Adv. Ind. Biotechnol. 2, 1–5 (2019).
5. Chromatography, A. A OVERVIEW ON AFFINITY CHROMATOGRAPHY: A REVIEW Anusha, Shyamala, P.Sirisha. 8, (2018).
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