Comparing Two Density Methods of Semen Preparation in Human Ejaculates

Abstract

Test tube baby is always a fascinating field of human reproductive biological science playing an important role in treating infertility. Poor semen quality is the major cause of infertility in human beings. Preparation of semen samples for intrauterine insemination (IUI) or in-vitro fertilization (IVF) is a key point in the success of test-tube embryo production. The study compared two different methods of semen preparation viz. double density gradient (DDG) and single density gradient (SDG) in patients seeking IUI / IVF treatment with their consent and permission of the hospital. Semen ejaculate from patients (n=100) was divided equally into two equal volume parts. One half of each sample was treated with DDG and another half with the SDG method of semen preparation. Results showed that sperm concentration was significantly higher (P>0.05) in SDG compared to DDG treated samples which were 58.65±181 and 49.89±180 Million/ml, respectively. Sperm motility of type-a and type-b both were significantly higher (P>0.05) in SDG compared to DDG treated samples which were 91.85± 3.15 and 68.85 ± 26.15. It is concluded that the single density gradient method is better than the double density gradient for semen ejaculates preparation during the treatment of male infertility using the in-vitro fertilization technique.