Development and Validation of Reverse-phase High-performance Liquid Chromatography Method for Novel Synthetic Pyridine Derivative

Abstract

The study was focused toward synthesis, characterization and quantification of Pyridine derivative by Reverse Phase High Performance Liquid Chromatography method. The synthesis of novel pyridine derivative was carried out by condensation reaction in presence of inert catalyst K2CO3 and THF solvent. Characterization was done by I.R, 1H-NMR,and HPLC. Based on the spectral data, the structure of novel pyridine derivative was characterized as 5 - (4 - Substituted amino) - 3- Nitrobenzene - 1 - Sulfonyl) - 4, 5, 6, 7 –Tetrahydrothieno[3, 2 - C] Pyridine The method validation including precision, accuracy, LOD, robustness, Linearity, and range was developed as per ICH guideline through an efficient isocratic RP-HPLC.It was determined that the above procedure is specified, accurate, precise, robust, and sturdy.