Çiğ Süt ve Dondurmadan İzole Edilen Staphylococcus aureus Suşlarının Metisilin Direnci ve Panton Valentine Toksini Üzerine Araştırma: Multiplex PCR ile Moleküler Çalışma

Abstract

Staphylococcus aureus is a very important pathogenic bacterium that causes nosocomial and community-acquired infections in humans, and is also one of the leading pathogens that causes food-borne poisoning. The presence of S. aureus in raw milk and dairy products, and especially the presence of MRSA (Methicillin Resistance S. aureus) strains, poses a potential risk to public health. The aim of this study was to investigate the presence of methicillin resistance and Panton-Valentine Leucocidin (PVL) toxin in Staphylococcus aureus isolated and identified from raw milk and ice cream in Konya (Turkey) by multiplex PCR method. A total of 55 S. aureus were isolated 49 (18%) from 260 raw milk samples collected from various farms and 6 (4%) from 150 ice cream samples sold in patisseries. The obtained isolates were identified as S. aureus with conventional and genotypic methods. Multiplex PCR was performed to detect the 16S rRNA, mecA, femA and lukS genes. While no mecA gene was detected in any of the 49 S. aureus isolates obtained from raw milk samples, the presence of mecA gene was observed in one of the 6 S. aureus isolates isolated from ice cream samples. The PVL gene was not detected in any of the S. aureus isolates studied. S. aureus contamination is common in raw milk samples and ice cream samples. In order to avoid this, it is necessary to comply with the hygiene conditions and increase the precautions even more.