Topraktan İzole Edilen Bacillus sp. Suşlarının Lipid Üretim Kapasitelerinin Taranması ve Farklı Boyama Teknikleri ile Lipid Boyama-Reference-Cited by-同舟云学术

Topraktan İzole Edilen Bacillus sp. Suşlarının Lipid Üretim Kapasitelerinin Taranması ve Farklı Boyama Teknikleri ile Lipid Boyama

Published:2023-10-27 Issue:5 Volume:23 Page:1094-1102
ISSN:2149-3367
Container-title:Afyon Kocatepe University Journal of Sciences and Engineering
language:tr
Short-container-title:

Author:

DEMİRKAN Elif¹^ORCID,YILDIRIM İrem¹^ORCID

Affiliation:

1. Bursa Uludağ Üniversitesi

Abstract

Microbial lipids have been attracting more and more attention in recent years as promising raw materials for the production of biodiesel and value-added compounds. In the current economic and environmental situation, finding new renewable sources of lipids will be crucial. Recent research has necessitated the search for new efficient microbial producers with lipid production efficiency. For this purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid content was reached by E226 with 54.5%. Bacillus sp. E40 lipid content was determined as 46.4%. In addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid producing capacity.

Publisher

Afyon Kocatepe Universitesi Fen Ve Muhendislik Bilimleri Dergisi

Subject

General Engineering

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