Author:
Sergides M.,Perego L.,Galgani T.,Arbore C.,Pavone F. S.,Capitanio M.
Abstract
AbstractCells sense mechanical signals and forces to probe the external environment and adapt to tissue morphogenesis, external mechanical stresses and a wide range of diverse mechanical cues. Here, we propose a combination of optical tools to manipulate single cells and measure the propagation of mechanical and biochemical signals inside them. Optical tweezers are used to trap microbeads that are used as handles to manipulate the cell plasma membrane; genetically encoded FRET-based force sensors inserted in F-actin and alpha-actinin are used to measure the propagation of mechanical signals to the cell cytoskeleton, while fluorescence microscopy with single-molecule sensitivity can be used with a huge array of biochemical and genetic sensors. We describe the details of the setup implementation, the calibration of the basic components and preliminary characterization of actin and alpha-actinin FRET-based force sensors.
Funder
Fondazione Cassa di Risparmio di Firenze
H2020 Research Infrastructures
Università degli Studi di Firenze
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy
Cited by
5 articles.
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