DNA Extraction from Blood Stored on FTA Cards

Author:

Elmrghni Samir

Abstract

Informed consent was obtained from 238 unrelated Libyan individuals (Benghazi region). DNA was extracted from blood stains collected on FTA1 cards About 1.2 mm FTA1 disc and 1 ng of DNA purified from buccal swabs was used products were separated and detected using the ABI Prism 310xl Genetic Analyzer (Applied Biosystems) according to the manufacturer’s recommended protocol. The data were analysed using GeneMapper ID v3.2 (Applied Biosystems). Quality control - The laboratory has participated in the Y-STR Haplotyping Quality Assurance Exercise (Certified at 2010-5-20). We conclude that amplification of DNA stored on FTA cards can be used safely to analyse DNA profiles.

Publisher

AMO Publisher

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