Molecular Detection of Influenza A(H1N1)Pdm09 Virus Among Chronic Kidney Disease Patients: A Peripheral Blood Sample Approach and Assessment of the Associated Risk Factors
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Published:2023-07-06
Issue:4
Volume:1
Page:583-589
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ISSN:2786-7447
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Container-title:European Journal of Theoretical and Applied Sciences
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language:
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Short-container-title:ejtas
Author:
Chiwar Hassan MusaORCID, Chiroma Fatima AliyuORCID, Isaac Agyigra AksavdwaORCID, Abubakar Mustapha BalaORCID, Oderinde Bamidele SojiORCID, Musa Aminu IsaORCID, Machina Idris BabaORCID, Loskurima Umar, Amuwa Nuhu AhmaduORCID
Abstract
Introduction: Chronic kidney disease (CKD) is a progressive loss of functional nephron characterized by various risk factors. Influenza virus has been found to cause rhabdomyolysis, which is toxic to the kidneys and can initiate or worsen CKD. This study aims to investigate the frequency and molecular detection of Influenza A(H1N1)Pdm09 Virus gene among CKD patients attending University of Maiduguri Teaching Hospital, Nigeria.Materials and Methods: Peripheral blood samples were collected from 150 CKD patients. One-step RT-PCR was performed for detection of influenza virus using the Centers for Disease Control and Prevention protocol. Relevant clinical data were collected in standardized questionnaires from each patient, and medical history was obtained from their hospital records.Results: Conventional PCR analysis revealed that 16% of the CKD patients tested positive for Inf A/Pdm H1N1. The virus frequency was found to be higher among patients in CKD stage 5 (end-stage CKD) and lower in CKD stage 3 (moderate CKD). Additionally, female CKD patients and those in the age group of 55-64 years showed a higher susceptibility to Inf A/Pdm H1N1 infection.Discussion: The study provides evidence of the presence of Inf A/Pdm H1N1 in CKD patients, aligning with previous research showing its involvement in kidney disease aggravation. CKD patients often exhibit immune dysregulation, which might facilitate the virus's invasion and persistence. Conclusion: This study provides evidence of an association between Influenza A(H1N1)Pdm09 viraemia and decreased kidney function among CKD patients. The findings highlight the importance of monitoring and preventing influenza infection in CKD patients to prevent further kidney damage.
Reference16 articles.
1. Bagshaw, S. M., Sood, M. M., Long, J., Fowler, R. A., Adhikari, N. K., & Canadian Critical Care Trials Group H1N1 Collaborative (2013). Acute kidney injury among critically ill patients with pandemic H1N1 influenza A in Canada: cohort study. BMC nephrology, 14, 123. https://doi.org/10.1186/1471-2369-14-123 2. Bikbov, B., Perico, N., Remuzzi, G., & on behalf of the GBD Genitourinary Diseases Expert Group (2018). Disparities in Chronic Kidney Disease Prevalence among Males and Females in 195 Countries: Analysis of the Global Burden of Disease 2016 Study. Nephron, 139(4), 313–318. https://doi.org/10.1159/000489897 3. Choi, S. M., Xie, H., Campbell, A. P., Kuypers, J., Leisenring, W., Boudreault, A. A., Englund, J. A., Corey, L., & Boeckh, M. (2012). Influenza viral RNA detection in blood as a marker to predict disease severity in hematopoietic cell transplant recipients. The Journal of infectious diseases, 206(12), 1872–1877. https://doi.org/10.1093/infdis/jis610 4. Eleftheriadis, T., Antoniadi, G., Liakopoulos, V., Kartsios, C., & Stefanidis, I. (2007). Disturbances of acquired immunity in hemodialysis patients. Seminars in dialysis, 20(5), 440–451. https://doi.org/10.1111/j.1525-139X.2007.00283.x 5. Fernández-Fresnedo, G., Ramos, M. A., González-Pardo, M. C., de Francisco, A. L., López-Hoyos, M., & Arias, M. (2000). B lymphopenia in uremia is related to an accelerated in vitro apoptosis and dysregulation of Bcl-2. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 15(4), 502–510. https://doi.org/10.1093/ndt/15.4.502
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