Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs <i>Cimex lectularius</i> L. (Heteroptera: Cimicidae)-Reference-Cited by-同舟云学术

Metabolic resistance to deltamethrin is mediated by P450 and esterases in common bed bugs Cimex lectularius L. (Heteroptera: Cimicidae)

Published:2023-03-22 Issue:1 Volume:41 Page:11-16
ISSN:2054-930X
Container-title:Journal of the European Mosquito Control Association
language:en
Short-container-title:Journal of the European Mosquito Control Association

Author:

Cáceres M.¹,Drago A.²,Orihuela P.L. Santo¹³,Vassena C.V.¹⁴

Affiliation:

1. Centro de Investigaciones de Plagas e Insecticidas – UNIDEF – CONICET, San Juan Bautista de La Salle 4397, 1603 Villa Martelli, Buenos Aires, Argentina.

2. Entostudio S.R.L. Viale del Lavoro 66, 35020 Ponte San Nicolò, Italy.

3. Cátedra de Química Analítica Instrumental-Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954, 1113 Ciudad Autónoma de Buenos Aires, Argentina.

4. Instituto de Ingeniería e Investigaciones Ambientales, Universidad Nacional de San Martín, Campus Miguelete, 25 de Mayo y Francia, 1650 San Martín, Buenos Aires, Argentina.

Abstract

The infestations of Cimex lectularius L. (Heteroptera: Cimicidae) registered in the last decades have been influenced by several human activities, including international tourism and commerce. Moreover, the development of insecticide resistance and careless pest control strategies contributed to the dispersal of bed bugs. Given the complexity of the topic, distinguishing physiological and molecular mechanisms involved in resistance can help design proper control tools and limit the resistance spread. Here we determined the susceptibility to deltamethrin and imidacloprid in bed bugs collected in Italy. Also, we assessed the role of esterases and P450 monooxygenases by direct enzymatic activity measurement and inhibition by synergism bioassays. Our results showed that the field-collected colony exhibited high resistance ratios to imidacloprid and deltamethrin (757 and >60,000 times, respectively) compared to the susceptible colony. Moreover, resistant bed bugs showed increased activity of esterases and P450 monooxygenases. The synergistic effect of piperonyl butoxide (PBO) suggests the significant contribution of both enzymatic groups as detoxification pathways implicated in pyrethroid-resistant bed bugs. Further investigations are needed to unravel the biochemical and molecular basis involved in the resistant phenotype for developing novel strategies for pest control.

Publisher

Brill

Subject

General Medicine

Link

https://www.wageningenacademic.com/doi/pdf/10.52004/JEMCA2022.0003

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1. Publishing, meeting, and sharing data to go forward;Journal of the European Mosquito Control Association;2023-03-22