Crude extract of Jatobá leaves promotes canine osteosarcoma cell D17 proliferation
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Published:2022-05-24
Issue:
Volume:
Page:1283-1289
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Vieira V. S.1ORCID, Cruz V. S.2ORCID, Nepomuceno L. L.2ORCID, Soares N. P.1ORCID, Arnhold E.1ORCID, Teixeira W. F. P.1ORCID, Vieira D. S.3ORCID, Borges J. C. A.1ORCID, Paixão F. M.1ORCID, Araújo E. G.1ORCID
Affiliation:
1. Multi-User Laboratory for the Evaluation of Molecules Cells and Tissues, Veterinary and Zootechnical School, Federal University of Goiás, Campus Samambaia, Avenida Esperança, Goiânia, GO, 74690-900, Brazil. 2. Department of Veterinary Medicine, Catholic University Center of East Minas, Campus Coronel Fabriciano, Av. Pres. Tancredo de Almeida Neves, 3500, B - Morada do Vale B, Cel. Fabriciano MG, 35170-056, Brazil. 3. Embrapa Southeast Livestock, São Carlos, São Paulo, Brazil, Rod, Washington Luiz, Km 234 - Fazenda Canchim, São Carlos - SP, 13560-970, Brazil.
Abstract
Background and Aim: New substances for neoplasm treatment have to be carefully studied to minimize adverse effects and prevent disease progression stimulation. Jatobá is a typical tree of the Cerrado and Caatinga biome, with antifungal, antimicrobial, larvicide, antioxidant, and antiproliferative properties. This study aimed to investigate the action of the crude extract of Jatobá leaves (EBFJ) on canine osteosarcoma (CO) cells and analyze the expression of biomarkers in neoplasm progression.
Materials and Methods: D17 cells were cultured and subjected to treatment with EBFJ at different concentrations (10 μg/ mL; 100 μg/mL; 1000 μg/mL; 2000 μg/mL; and 5000 μg/mL) and exposure times (24 h, 48 h, and 72 h). The tetrazolium reduction assay and the immunocytochemistry technique, with anti-Bcl2, anti-p53, and anti-Ki-67 antibodies, were used to observe the effect of the extract on cell proliferation.
Results: Doses of 2000 μg and 5000 μg had cell viability of 300.80% and 361.84%, respectively. The extract did not show significant cytotoxicity of samples with the control group. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with EBFJ, with a statistical difference from the group without treatment.
Conclusion: EBFJ was not cytotoxic and had a proliferative effect on CO D17 cells. The confluence of cells, the number of labeled cells, and the expression of Bcl2, Ki-67, and p53 were higher in the groups treated with the extract.
Funder
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Conselho Nacional de Desenvolvimento Científico e Tecnológico Fundação de Amparo à Pesquisa do Estado de Goiás
Publisher
Veterinary World
Subject
General Veterinary
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