Cervical anticancer activities of Annona squamosa Linn. leaf isolate

Author:

Swantara Made Dira1ORCID,Rita Wiwik Susanah1ORCID,Dira Made Asmarani2ORCID,Agustina Kadek Karang3ORCID

Affiliation:

1. Department of Applied Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University, Denpasar, Bali 80225 Indonesia.

2. Clinical and Community Pharmacy Study Program, Faculty of Health, Bali Institute of Technology and Health, Denpasar, Bali 80225, Indonesia.

3. Department of Public Health, The Faculty of Veterinary Medicine, Udayana University, Denpasar, Bali 80225, Indonesia.

Abstract

Background and Aim: Cancer is one of the leading causes of death, the need for new anticancer herbal drugs is becoming more urgent considering the side effects of synthetic drugs. This study aimed to determine the anticancer activity of isolates derived from the methanol extract of Annona squamosa Linn. leaves and to identify the compounds that have an active effect against HeLa cells. Materials and Methods: The leaf metabolites of A. squamosa L. were extracted using methanol at room temperature (28°C) and were partitioned into n-hexane, chloroform, and n-butanol. The toxicity test of these extracts was conducted using a brine shrimp lethality assay. Furthermore, the most toxic extracts were separated and purified using silica gel column chromatography to yield four isolate fractions: FA, FB, FC, and FD. The most toxic isolates were tested for anticancer against HeLa cells, and their compounds were identified using liquid chromatography-mass spectrometry. Results: The results showed that the most toxic isolate with an LC50 value of 100.00 ppm had a potency similar to that of an anticancer agent with an IC50 value of 70.9021 ppm. Furthermore, the five compounds identified in this isolate include (6S, 7aR)-6-hydroxy-4,4,7a-trimethyl-6,7-dihydro-5H-1-benzofuran-2-one or loliolide, cocamidopropyl betaine, N-[3- (dimethylamino)propyl]dodecanamide or lauramidopropyl dimethylamine, linolenic acid, and 1-dodecyl-2-azepanone or laurocapram. Conclusion: It can be concluded that the leaf isolates of A. squamosa Linn. had shown anticancer activities against cervical cancer.

Funder

Universitas Udayana

Publisher

Veterinary World

Subject

General Veterinary

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