Affiliation:
1. Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
Abstract
Aim: The present study was carried out to determine the prevalence and molecular detection of fluoroquinolone-resistant Escherichia coli carrying qnrA and qnrS genes in healthy broiler chickens in Mymensingh, Bangladesh, and also to identify the genes responsible for such resistance.
Materials and Methods: A total of 65 cloacal swabs were collected from apparently healthy chickens of 0-14 days (n=23) and 15-35 days (n=42) old. The samples were cultured onto Eosin Methylene Blue Agar, and the isolation and identification of the E. coli were performed based on morphology, cultural, staining, and biochemical properties followed by polymerase chain reaction (PCR) targeting E. coli 16S rRNA genes. The isolates were subjected to antimicrobial susceptibility test against five commonly used antibiotics under fluoroquinolone (quinolone) group, namely gatifloxacin, levofloxacin, moxifloxacin, ofloxacin, and pefloxacin by disk diffusion method. Detection of qnrA and qnrS genes was performed by PCR.
Results: Among the 65 cloacal samples, 54 (83.08%) were found to be positive for E. coli. Antibiotic sensitivity test revealed that, of these 54 isolates, 18 (33.33%) were found to be resistant to at least one fluoroquinolone antibiotic. The highest resistance was observed against pefloxacin (61.11%). By PCR, of 18 E. coli resistant to fluoroquinolone, 13 (72.22%) were found to be positive for the presence of qnrS. None of the isolates were found positive for qnrA.
Conclusion: Fluoroquinolone-resistant E. coli harboring qnrS genes is highly prevalent in apparently healthy broiler chickens and possesses a potential threat to human health.
Funder
Bangladesh Agricultural Research Institute
Reference24 articles.
1. Institute of Medicine. (2010) Antibiotic Resistance: Implications for Global Health and Novel Intervention Strategies. The National Academies Press, Washington, DC.
2. Chika, E., Charles, E., Ifeanyichukwu, I. and Michael, A. (2018) First detection of fox-1 AmpC β-lactamase gene expression among Escherichia coli isolated from abattoir samples in Abakaliki, Nigeria. Oman Med. J., 33(3): 243-249.
3. Todar, K. (2007) Pathogenic E. coli. Online Textbook for Bacteriology. Available from: http://www.textofbacteriology.net/e.coli.html. Accessed on 18-06-2009.
4. Gross, W.G. (1994) Diseases due to Escherichia coli in poultry. In: Gyles, C.L., editor. Escherichia coli in Domestic Animals and Humans. CAB International, Wallingford, United Kingdom. p237-259.
5. Hricová, M., Röderová, M., Pudová, V., Hanulík, V., Halová, D., Julínková, P., Dolejská, M., Papoušek, I. and Bardoň, J. (2017) Quinolone-resistant Escherichia coli in poultry farming. Cent. Eur. J. Public Health, 25(2): 163-167.
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