Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats-Reference-Cited by-同舟云学术

Association of the KiSS1 gene with litter size in Cyprus and Iraqi black goats

Published:2021-08-03 Issue: Volume: Page:1995-2001
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Rahawy M. A.¹^ORCID,AL-Mutar Hayder Abdul-Kareem²^ORCID

Affiliation:

1. Department of Surgery and Theriogenology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.

2. Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Iraq.

Abstract

Aim: The study investigated the genetic polymorphism of the kisspeptin (KiSS1) gene and its relationship with litter size in Cyprus and Iraqi black goats. Materials and Methods: Blood samples (n=124) were collected from the two goat breeds reared at the Agricultural Research-Ruminant Research Station Breeding Station, Baghdad, Iraq. Genomic DNA was isolated using a DNA extraction kit. Polymerase chain reaction (PCR) was used to amplify the KiSS1 gene. All PCR products were sequenced and samples were used for further analysis using NCBI-Blast online on the exon 1 (595 bp) region of the KiSS1 gene. Results: The results of this study revealed a significantly (P<0.05) larger litter size of the Cyprus goat breed than in the Iraqi black goats in the first and second parity. Three (893G/C, 973C/A, and 979T/G) substitutions relative to the KiSS1 gene reference sequence (GenBank ID: J × 047312.1, KC989928.1) were identified. Only the mutation g893G>C was identified as a single nucleotide polymorphism (SNP) associated with litter size. Furthermore, the average alleles in KiSS1 gene of both types of goats 0.567 and 0.3715 GG, were recorded. The genotyping at locus g893C>G was demonstrating domination of fecundity quality litter size, Both genotypes SNP of GC were classified at this marked region of KiSS1 gene. Conclusion: The study concluded that the role of the KiSS1 gene in fecundity, revealing the status of this gene as an indicator in the assisted of caprine breeding selection.

Publisher

Veterinary World

Subject

General Veterinary

Link

http://www.veterinaryworld.org/Vol.14/August-2021/2.pdf

Reference31 articles.

1. Deldar-Tajangookeh, H., Shahneh, A.Z., Zamiri, M.J., Daliri, M., Kohram, H. and Nejati-Javaremi, A. (2009) Study of BMP-15 gene polymorphism in Iranian goats. Afr. J. Biotechnol., 8(13): 2929-2932.

2. Al-Mutarm, H. (2018) Molecular and physiological role of BMP15 gene associated with ovulation rate and fertility of Iraq goat. J. Anim. Vet. Adv., 17(5): 104-110.

3. An, X., Ma, T., Hou, J., Fang, F., Han, P., Yan, Y., Zhao, H., Song, Y., Wang, J. and Cao, B. (2013) Association analysis between variants in KISS1 gene and litter size in goats. BMC Genet., 14(63): 1-6

4. Othman, O.E., Darwish, H.R., Abou-Eisha, A., El-Din, A.E. and Abdel-Samad, M.F. (2015) DNA characterization and polymorphism of KISS1 gene in Egyptian small ruminant breeds. Afr. J. Biotechnol., 14(30): 2335-2340.

5. Messager, S., Chatzidaki, E.E., Ma, D., Hendrick, A.G., Zahn, D., Dixon, J., Thresher, R.R., Malinge, I., Lomet, D., Carlton, M.B., Colledge, W.H., Caraty, A. and Aparicio, S.A. (2005) Kisspeptin directly stimulates gonadotropin releasing hormone release via G protein-coupled receptor 54. Proc. Natl. Acad. Sci. U. S. A., 102(5): 1761-1766.