Interepidemic xenosurveillance of Japanese encephalitis virus and Zika virus in Culex mosquitoes from Ubon Ratchathani province, Thailand-Reference-Cited by-同舟云学术

Interepidemic xenosurveillance of Japanese encephalitis virus and Zika virus in Culex mosquitoes from Ubon Ratchathani province, Thailand

Published:2024-07 Issue: Volume: Page:1555-1561
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Surasa Wilasinee¹^ORCID,Pientong Chamsai²^ORCID,Ekalaksananan Tipaya²^ORCID,Overgaard Hans Jorgen³^ORCID,Aromseree Sirinart²^ORCID,Phanthanawiboon Supranee¹^ORCID

Affiliation:

1. Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

2. Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; HPV&EBV and Carcinogenesis, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.

3. Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Medical Entomologist, Faculty of Science and Technology, Norwegian University of Life Sciences, Ås, Norway.

Abstract

Background and Aim: Some Culex mosquitoes are competent vectors for Japanese encephalitis virus (JEV) and Zika virus (ZIKV), which cause public health problems worldwide, especially in South-east Asia. Xenosurveillance of Culex mosquitoes remains limited compared with other common mosquito-borne diseases. This study aimed to identify JEV and ZIKV in field-caught Culex mosquitoes collected from Ubon Ratchathani province. Materials and Methods: We investigated the presence of JEV and ZIKV in Culex mosquitoes from two districts in Ubon Ratchathani province, Thailand, and examined their role in viral interepidemic circulation. Female Culex mosquitoes (5,587) were collected using a mechanical aspirator from indoors and outdoors. The consensus sequences of the E and NS1 genes of JEV and the E gene of ZIKV were identified using real-time reverse transcription-polymerase chain reaction. Results: From 335 sample pools that contain a total of 5587 adult female Culex mosquitoes collected from Don Yung, Mueang district (4,406) and Phon Duan, Det Udom district (1,181), none of the collected mosquitoes tested positive for either JEV or ZIKV. Conclusion: This study did not find JEV and ZIKV in Culex mosquitoes collected from the area of collection, which may be due to the low circulating amount of the virus in the vectors in the area, making it undetectable, or it may be because Culex mosquitoes are not suitable vector for the virus being tested. However, further xenosurveillance study of JEV and ZIKV in mosquito is suggested to prepare for the next outbreak. Keywords: Culex, Japanese encephalitis virus, Thailand, xenosurveillance, Zika.

Funder

Norges Forskningsråd

Publisher

Veterinary World

Reference27 articles.

1. Solomon, T., Ni, H., Beasley, D.W., Ekkelenkamp, M., Cardosa, M.J. and Barrett, A.D. (2003) Origin and evolution of Japanese encephalitis virus in Southeast Asia. J. Virol., 77(5): 3091–3098.

2. Olsen, S.J., Supawat, K., Campbell, A.P., Anantapreecha, S., Liamsuwan, S., Tunlayadechanont, S., Visudtibhan, A., Lupthikulthum, S., Dhiravibulya, K., Viriyavejakul, A., Vasiknanonte, P., Rajborirug, K., Watanaveeradej, V., Nabangchang, C., Laven, J., Kosoy, O., Panella, A., Ellis, C., Henchaichon, S., Khetsuriani, N., Powers, A.M., Dowell, S.F. and Fischer, M. (2010) Japanese encephalitis virus remains an important cause of encephalitis in Thailand. Int. J. Infect. Dis., 14(10): e888–e892.

3. Sudjaritruk, T., Kaewpoowat, Q., Prasarakee, C., Sarachai, S., Taurel, A.F., Sricharoen, N., Assawawongprom, P., Saheng, J., Harris, R., Nealon, J. and Yoksan, S. (2022) Seroepidemiological study of Japanese encephalitis virus in Chiang Mai: Immunity and susceptibility 28 years after introduction of a vaccination programme. PLoS Negl. Trop. Dis., 16(8): e0010674.

4. Bureau of Epidemiology DODC. and Ministry of Public Health of Thailand. National Disease Surveillance Report 506; Japanese B Encephalitis. Available from: http://doe.moph.go.th/surdata/disease.php?dcontent=old&ds=29. Retrieved on 06-07-2024.

5. Nitatpattana, N., Dubot-Pérès, A., Gouilh, M.A., Souris, M., Barbazan, P., Yoksan, S., De Lamballerie, X. and Gonzalez, J.P. (2008) Change in Japanese encephalitis virus distribution, Thailand. Emerg. Infect. Dis., 14(11): 1762–1765.