Medical prospects of cryptosporidiosis in vivo control using biofabricated nanoparticles loaded with Cinnamomum camphora extracts by Ulva fasciata-Reference-Cited by-同舟云学术

Medical prospects of cryptosporidiosis in vivo control using biofabricated nanoparticles loaded with Cinnamomum camphora extracts by Ulva fasciata

Published:2024-01 Issue: Volume: Page:108-124
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Allam Nesreen Allam Tantawy¹^ORCID,Hamouda Ragaa Abd El-Fatah²^ORCID,Sedky Doaa¹^ORCID,Abdelsalam Mahinour Ezzeldin³^ORCID,El-Gawad Mona Ebrahim Hussien Abd⁴^ORCID,Hassan Noha Mahmoud Fahmy¹^ORCID,Aboelsoued Dina¹^ORCID,Elmaaty Amal M. Abou⁵^ORCID,Ibrahim Muhammad A.⁴^ORCID,Taie Hanan Anwar Aly⁶^ORCID,Hakim Ashraf Samir⁷^ORCID,Desouky Hassan Mohamed⁵^ORCID,Megeed Kadria Nasr Abdel¹^ORCID,Abdel-Hamid Marwa Salah²^ORCID

Affiliation:

1. Department of Parasitology and Animal Diseases, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.

2. Department of Microbial Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, 5th Zone, Sadat City, Munofia, Egypt.

3. Department of General Biology, Center of Basic Sciences, Misr University for Science and Technology, Al Motamayez District, 6th of October, Giza, Cairo, Egypt.

4. Cytogenetics and Animal Cell Culture Lab., National Gene Bank, Agriculture Research Center, 9 Gamaa Street, Giza, Cairo, Egypt.

5. Department of Animal Reproduction and Artificial Insemination, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.

6. Department of Plant Biochemistry, Agriculture and Biological Researches Institute, National Research Centre, 33 El-Bohouth St. (Former El-Tahrir St.), Dokki, P.O. 12622, Giza, Cairo, Egypt.

7. Department of Microbiology and Immunology, Veterinary Research Institute, National Research Centre, 33 El Buhouth Street, Dokki, P.O. Box: 12622, Giza, Cairo, Egypt.

Abstract

Background and Aim: Global efforts are continuing to develop preparations against cryptosporidiosis. This study aimed to investigate the efficacy of biosynthesized Ulva fasciata loading Cinnamomum camphora oil extract on new zinc oxide nanoparticles (ZnONPs shorten to ZnNPs) and silver nanoparticles (AgNPs) as alternative treatments for Cryptosporidium parvum experimental infection in rats. Materials and Methods: Oil extract was characterized by gas chromatography-mass spectrometry, loaded by U. fasciata on ionic-based ZnO and NPs, and then characterized by transmission electron microscopy, scanning electron microscopy, and X-ray diffraction. Biosafety and toxicity were investigated by skin tests. A total of 105 C. parvum oocysts/rat were used (n = 81, 2–3 W, 80–120 g, 9 male rats/group). Oocysts shedding was counted for 21 d. Doses of each preparation in addition to reference drug were administered daily for 7 d, starting on post-infection (PI) day (3). Nitazoxanide (100 mg) was used as the reference drug. After 3 weeks, the rats were sacrificed for postmortem examination and histopathological examination. Two blood samples/rat/group were collected on the 21st day. Ethylenediaminetetraacetic acid blood samples were also used for analysis of biochemistry, hematology, immunology, micronucleus prevalence, and chromosomal abnormalities. Results: C. camphora leaves yielded 28.5 ± 0.3 g/kg oil and 20 phycocompounds were identified. Spherical and rod-shaped particles were detected at 10.47–30.98 nm and 18.83–38.39 nm, respectively. ZnNPs showed the earliest anti-cryptosporidiosis effect during 7–17 d PI. Other hematological, biochemical, immunological, histological, and genotoxicity parameters were significantly fruitful; hence, normalized pathological changes induced by infestation were observed in the NPs treatments groups against the infestation-free and Nitazoxanide treated group. Conclusion: C. camphora, U. fasciata, ZnNPs, and AgNPs have refluxed the pathological effects of infection as well as positively improved host physiological condition by its anticryptosporidial immunostimulant regenerative effects with sufficient ecofriendly properties to be proposed as an alternative to traditional drugs, especially in individuals with medical reactions against chemical commercial drugs. Keywords: blood biomarkers, Cinnamomum camphora, Cryptosporidium parvum, cytokines, Egypt, genotoxicity, green nanoparticles, rats, Ulva fasciata.

Funder

National Research Centre

Publisher

Veterinary World

Link

https://www.veterinaryworld.org/Vol.17/January-2024/12.pdf

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