Affiliation:
1. Stress and Oxidative Stress in Animal Research Unit, Faculty of Veterinary Sciences, Mahasarakham University, Mahasarakham 44000, Thailand.
Abstract
Background and Aim: Ovariohysterectomy (OHE) is a common procedure for sterilization of female dogs. However, knowledge of changes in pain stress, oxidative stress, and total antioxidant power status before, during, and after OHE is limited. The objective of this experiment was to study the effect of duration on pain stress, oxidative stress, and total antioxidant power status in female dogs undergoing OHE.
Materials and Methods: Seven female dogs were sterilized using the OHE method. Pain scores, hematological changes, and biochemical markers were investigated during pre-operative, 3 h after starting OHE, and on days 3, 7, 10, and 14 of an experimental period. Data were analyzed using one-way analysis of variance.
Results: At 3 days after OHE, pain score was higher than on days 7-14 of the experimental period; percentage of neutrophil, 3 h after starting OHE, was higher than during pre-operative and on days 3-14 of the experimental period; percentage of lymphocyte on days 10-14 was lower than during pre-operative, 3 h after starting OHE, and on days 3-7 of the experimental period; neutrophil/lymphocyte ratio, 3 h after starting OHE, was higher than during pre-operative and on days 3-14 of the experimental period; plasma malondialdehyde on day 3 was higher than during pre-operative, 3 h after starting OHE, and on days 3-14 of the experimental period; and total antioxidant power on day 14 was higher than during pre-operative, 3 h after starting OHE, and on days 3-10 of the experimental period, respectively.
Conclusion: This experiment indicated that OHE caused pain stress, oxidative stress, and reduction of total antioxidant power in female dogs. Finally, female dogs needed antioxidant for 7 days after OHE.
Reference24 articles.
1. Arunkumar, S., Dilipkumar, D., Shivaprakash, B.V. and Bhagvantappa. (2017) Comparison of right flank and ventral midline approach for ovariohysterectomy in dog. J. Entomol. Zool. Stud., 5(6): 2411-2416.
2. Jarolmasjed, S. and Alizadeh, F. (2016) Evaluation of the effectiveness of uterine hook application in large breed canine ovariohysterectomy: Physiological study, Iran. J. Vet. Surg., 11(2): 31-38.
3. Tallant, A., Ambros, B., Freire, C. and Sakals, S. (2016) Comparison of intraoperative and postoperative pain during canine ovariohysterectomy and ovariectomy. Can. Vet. J., 57(7): 741-746.
4. Kisani, A.I., Tughgba, T. and Elsa, A.T. (2018) Effects of various surgical procedures on biochemical parameters of Nigerian dogs and their clinical implications. Vet. World, 11(7): 909-914.
5. Hoglund, O.V., Lovebrant, J., Olsson, U. and Hoglund, K. (2016) Blood pressure and heart rate during ovariohysterectomy in pyometra and control dogs: A preliminary investigation. Acta Vet. Scan., 58(1): 80.