Epidemiological and molecular studies on Echinococcus granulosus from free-roaming dogs in Southeast Iran
-
Published:2020
Issue:4
Volume:13
Page:739-745
-
ISSN:2231-0916
-
Container-title:April-2020
-
language:en
-
Short-container-title:Vet World
Author:
Keyhani Alireza1, Sharifi Iraj2ORCID, Bamorovat Mehdi3, Mohammadi Mohammad Ali4ORCID, Askari Asma5, Ebrahimipour Mohammad4ORCID, Harandi Majid Fasihi4ORCID
Affiliation:
1. Student Research Committee, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran. 2. Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran. 3. Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran. 4. Research Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, Iran. 5. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Abstract
Background and Aim: Cystic echinococcosis (CE), as a major zoonotic helminth infection, imposes remarkable socioeconomic burden on many endemic countries across the world, including Iran. Due to the high importance of free-roaming dogs in the transmission of CE, epidemiological and molecular studies in this type of hosts are required in the endemic regions. This study aimed to investigate the epidemiology and genotyping of Echinococcus granulosus isolated from stray dogs in Kerman, Southeast Iran.
Materials and Methods: Eighty-four samples were isolated from stray dogs in the city and suburbs of Kerman in coordination with the health authorities and the municipality office for rabies control and dog population management. Dog demographic data, including age and sex were collected. The worm was isolated by necropsy and genomic DNA was extracted and partial cytochrome c oxidase subunit 1 gene was amplified using specific primers. Phylogenetic and Templeton-Crandall-Sing (TCS) network analyses were carried out on the sequence data.
Results: The overall prevalence of CE in the surveyed dogs was 10.7% (9/84 cases). Out of 84 stray dogs, 33 (39.3%) and 51 (60.7%) cases were male and female, respectively. There was not a statistically significant difference between the infection and gender of dogs. However, infection is shown more in dogs under one year of age with a statistically significant difference (p<0.05). The results of molecular studies indicated E. granulosus G1 genotype for all isolates. The high presence of free-roaming dogs in urban and peri-urban areas and high frequency of parasite in this animal is a risk factor for humans in the region. Haplotype sequence analysis on the dog isolates revealed a close relationship with other E. granulosus isolates in Kerman.
Conclusion: The findings of this study provide evidence-based data about the epidemiological and molecular characteristics of CE in dog definitive hosts of Southeast Iran. Further studies are required to understand the prevalence and parasite genotypes in dogs in Iran.
Funder
Kerman University of Medical Sciences
Publisher
Veterinary World
Subject
General Veterinary
Reference55 articles.
1. Dabaghzadeh, H., Bairami, A., Beigom, K.E., Aryaeipour, M., and Rokni, M.B. (2018) Seroprevalence of human cystic echinococcosis in Alborz Province, central Iran in 2015. Iran. J. Public Health, 47(4): 561. 2. Deplazes, P., Rinaldi, L., Rojas, C.A., Torgerson, P.R., Harandi, M.F., Romig, T., Antolova, D., Schurer, J.M., Lahmar, S., Cringoli, G. and Magambo, J. (2017) Global distribution of alveolar and cystic echinococcosis. Adv. Parasitl., 95(2): 315-493. 3. Harandi, M.F., Budke, C.M. and Rostami, S. (2012) The monetary burden of cystic echinococcosis in Iran. PLoS Negl. Trop. Dis., 6(11): e1915. 4. Mirbadie, S., Kamyabi, H., Mohammadi, M., Shamsaddini, S. and Harandi, M. (2018) Copro-PCR prevalence of Echinococcus granulosus infection in dogs in Kerman, South-Eastern Iran. J. Helminthol., 92(1): 17-21. 5. Moro, P. and Schantz, P.M. (2009) Echinococcosis: A review. Int. J. Infect. Dis., 13(2): 125-133.
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|