The first study on antimicrobial resistance of Staphylococcus aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, East Java, Indonesia
-
Published:2023-04
Issue:
Volume:
Page:786-791
-
ISSN:2231-0916
-
Container-title:Veterinary World
-
language:en
-
Short-container-title:Vet World
Author:
Praja Ratih Novita1ORCID, Yudhana Aditya2ORCID, Saputro Amung Logam3ORCID, Hamonangan Jonathan Mark4ORCID
Affiliation:
1. Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia. 2. Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia. 3. Veterinary Medicine Study Program, Department of Health and Life Sciences, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia; Department of Veterinary Science, Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga, Kampus C Mulyorejo Street, Surabaya, East Java, Indonesia. 4. Veterinary Medicine Study Program, School of Health and Life Sciences, Universitas Airlangga, Wijaya Kusuma Street 113, Banyuwangi, East Java, Indonesia.
Abstract
Background and Aim: Raw goat milk is a highly nutritious dairy product and a suitable medium for the growth of antimicrobial-resistant Staphylococcus aureus, the leading cause of subclinical mastitis. This study aimed to investigate the resistance status of S. aureus which isolated from goat milk associated with subclinical mastitis cases in Siliragung Subdistrict, Banyuwangi District, East Java, Indonesia.
Materials and Methods: The S. aureus isolates were recovered from 258 raw goat milk samples from seven different dairy goat farms. Preliminary screening of subclinical mastitis using the California mastitis test, then samples with score +3 and +4 were taken for further isolation and identification, followed by a biochemical test to determine the S. aureus. Moreover, the bacteria susceptibility test against several antimicrobials was done using the disk diffusion method.
Results: Based on our findings, a total of 66 (25.58%) raw goat milk samples were tested positive for S. aureus, of which 36.36% were identified as multidrug-resistant. Moreover, S. aureus were also identified as resistant to penicillin (81.82%), ampicillin (65.15%), erythromycin (50.52%), and gentamicin (36.09%).
Conclusion: The prevalence of S. aureus isolated from raw goat milk associated with subclinical mastitis in Siliragung Subdistrict, Banyuwangi District, Indonesia, was recorded at 25.58%. Moreover, 36.36% of S. aureus isolates were categorized as resistant to three or more classes of antibiotics. The biosafety and biosecurity procedures during the milking process should be strengthened in dairy goat farms to prevent the transmission of antimicrobial resistance among animals, humans, and environments.
Keywords: antimicrobial resistance, food security, infectious disease, public health, tropical disease.
Funder
Universitas Airlangga
Publisher
Veterinary World
Subject
General Veterinary
Reference35 articles.
1. Martínez, S.P.M., Romero, R.A.R., Olivares, R.A.C., Ortiz, V.E.E. and Watty, A.E.D. (2018) Identification of and antimicrobial resistance in bacteria causing caprine mastitis in three states and a city in Central Mexico under manual and mechanical milking conditions. J. Dairy Vet. Anim. Res., 7(3): 115–118. 2. Wang, W., Baloch, Z., Jiang, T., Zhang, C., Peng, Z., Li, F., Fanning, S., Ma, A. and Xu, J. (2017) Enterotoxigenicity and antimicrobial resistance of Staphylococcus aureus isolated from retail food in China. Front. Microbiol., 8 (2256): 1–11. 3. Khalil, I.I., Aldabbagh, S.Y.A. and Shareef, A.M. (2018) Isolation, identification and detection of some virulence factors in yeasts from local cheese in Mosul city. Iraqi. J. Vet. Sci., 32(1): 81–85. 4. Tyasningsih, W., Ramandinianto, S.C., Ansharieta, R., Witaningrum, A.M., Permatasari, D.A., Wardhana, D.K., Effendi, M.H. and Ugbo, E.N. (2022) Prevalence and antibiotic resistance of Staphylococcus aureus and Escherichia coli isolated from raw milk in East Java, Indonesia. Vet. World, 15(8): 2021–2028. 5. Regasa, S., Mengistu, S. and Abraha, A. (2019) Milk safety assessment, isolation, and antimicrobial susceptibility profile of Staphylococcus aureus in selected dairy farms of Mukaturi and Sululta Town, Oromia Region, Ethiopia. Vet. Med. Int., 2019(1): 3063185.
|
|