Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs-Reference-Cited by-同舟云学术

Association of natural resistance-associated macrophage protein 1 polymorphisms with Salmonella fecal shedding and hematological traits in pigs

Published:2022-11-29 Issue: Volume: Page:2738-2743
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Suwannawong Nattariga¹^ORCID,Thumarat Uschara²^ORCID,Phongphanich Pitchayanipa¹^ORCID

Affiliation:

1. Animal Production Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Songkhla 90112, Thailand; Center of Excellence on Agricultural Biotechnology (AG-BIO/MHESI), Bangkok 10900, Thailand.

2. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla 90112, Thailand.

Abstract

Background and Aim: Natural resistance-associated macrophage protein 1 encoding gene (Nramp1) plays a role in immune response and disease resistance. This study aimed to investigate the polymorphisms of Nramp1 intron 6 concerning Salmonella shedding and hematological traits in pigs. Materials and Methods: A total of 40 commercial pigs (three-way Large White x Landrace x Duroc cross) were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and analyze the relationship between the polymorphisms of the Nramp1 gene and Salmonella fecal shedding and hematological parameters. Results: Nramp1 was shown to be polymorphic in these pigs. The Nramp1 gene has two alleles (A and B) and two genotypes (AB and BB). The BB genotype had a higher frequency than the AB genotype. A significant relationship between the BB genotype and the number of Salmonella in feces compared to the AB genotype (p < 0.05) on 7 days post-inoculation (DPI) was revealed in the association analysis. The single-nucleotide polymorphism at intron 6 in the Nramp1 gene was linked to white and red blood cells 2 and 7 DPI (p < 0.05). Conclusion: The Nramp1 gene was suggested by these findings to be potentially used as a molecular marker for the genetic selection of disease susceptibility in pig breeding.

Funder

Ministry of Higher Education, Science, Research and Innovation, Thailand

Publisher

Veterinary World

Subject

General Veterinary

Link

http://www.veterinaryworld.org/Vol.15/November-2022/26.pdf

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