Pathological, microscopic, and molecular diagnosis of paratuberculosis/John’s disease in naturally infected dromedary camel (Camelus dromedarius)
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Published:2023-06
Issue:
Volume:
Page:1277-1283
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
El Tigani-Asil El Tigani Ahmed1ORCID, Abdelwahab Ghada El Derdiri1ORCID, Abdu El Hadi Ahmed Mohamed2ORCID, Terab Abdelnasir Mohammed Adam1ORCID, Khalil Nasareldien Altaib Hussein1ORCID, Al Marri Zhaya Jaber Mohammed1ORCID, Yuosf Mohd Farouk1ORCID, Shah Asma Abdi Mohamed1ORCID, Khalafalla Abdelmalik Ibrahim1ORCID, Ishag Hassan Zackaria Ali1ORCID
Affiliation:
1. Biosecurity Affairs Division, Development and Innovation Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates. 2. Extension Services and Animal Health Division, Animal Wealth Sector, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab Emirates.
Abstract
Background and Aim: Paratuberculosis (PTB) or John’s disease is a chronic disease of ruminants impeding the reproduction and productivity of the livestock sector worldwide. Since there is a lack of pathological studies explaining the nature and development of the disease in camels, this study aimed to highlight the anatomopathological changes of PTB in camels, which may help in verifying and validating some diagnostic tests used to detect the etiology of the disease in camel tissues.
Materials and Methods: In August 2017, at Alselaa border’s Veterinary Clinic of Al Dhafra Region, Western Abu Dhabi, UAE, one imported culled she-camel of 2 years old was subjected to clinical, microscopic, and anatomopathological investigations along with real-time quantitative polymerase chain reaction (q-PCR) to confirm the infection and correlate between clinical signs and pathological lesions of the PTB in dromedary camels.
Results: Clinically, typical clinical signs compliant with the pathognomonic gross and histologic lesions of PTB were seen in naturally infected dromedary camel. As presumptive diagnosis microscopically, acid-fast coccobacillus bacterium clumps were demonstrated in direct fecal smears as well as in scraped mucosal and crushed mesenteric lymph node films, and in histopathological sections prepared from a necropsied animal and stained by Ziehl-Neelsen stain. Free and intracellular acid-fast clump phagosomes were further confirmed as Mycobacterium avium subsp. paratuberculosis by q-PCR.
Conclusion: Clinical signs and pathological lesions of paratuberculosis in a dromedary camel were found to be similar to those of the other susceptible hosts.
Keywords: acid-fast bacteria, dromedary camel, Mycobacterium, paratuberculosis.
Publisher
Veterinary World
Subject
General Veterinary
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