Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines-Reference-Cited by-同舟云学术

Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines

Published:2023-07 Issue: Volume: Page:1546-1551
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Lee Chae Eun¹^ORCID,Ikeda Jeong Hee¹^ORCID,Manongdo Mikaella Andrea M.¹^ORCID,Romerosa Dan Rica T.¹^ORCID,Sandalo-De Ramos Kristina Andrea C.¹^ORCID,Tanaka Tetsuya²^ORCID,Galay Remil L.¹^ORCID

Affiliation:

1. Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, College, Laguna 4031, Philippines.

2. Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto 1-21-24, Kagoshima 890-0065, Japan.

Abstract

Background and Aim: Bartonella and Borrelia are zoonotic vector-borne pathogens that can infect dogs and humans. Data on Bartonella and Borrelia in dogs in the Philippines are lacking. This study was conducted to validate previous reports and further investigate the occurrence of Bartonella and Borrelia spp. in cities of Metro Manila. Materials and Methods: A total of 182 canine blood samples were acquired with DNA using a commercial extraction kit from selected veterinary clinics in the cities of Metro Manila and Laguna, Philippines. The mammalian actin was amplified through polymerase chain reaction (PCR), followed by PCR assays targeting Bartonella gltA and Borrelia flaB. Further PCR assays targeting 16S of Borrelia and ospA and ospC of Borrelia burgdorferi were performed for those that showed flaB bands. Results: A positive band for gltA of Bartonella was observed in 9 (4.95%) samples, whereas a positive band for flaB of Borrelia was observed in 15 (8.24%) samples. Subsequent PCR assays for other genes of Borrelia were negative. Conclusion: These results confirmed the presence of Bartonella and warranted further investigation for the possible presence of other Borrelia species. Keywords: Bartonella, Borrelia, dogs, polymerase chain reaction, tick-borne pathogens.

Funder

Japan Society for the Promotion of Science

Heiwa Nakajima Foundation

Publisher

Veterinary World

Subject

General Veterinary

Link

https://www.veterinaryworld.org/Vol.16/July-2023/23.pdf

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