MicroRNA profiling in dogs undergoing induced ischemic heart infarction: An experimental study
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Published:2023-06
Issue:
Volume:
Page:1319-1324
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Raffee Liqaa A.1ORCID, Alawneh Khaled Z.2ORCID, Alshehabat Musa Ahmed Mohammed3ORCID, Haddad Hazem4ORCID, Jaradat Saied A.4ORCID
Affiliation:
1. Department of Accident and Emergency Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. 2. Department of Diagnostic Radiology and Nuclear Medicine, Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. 3. Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid, Jordan. 4. Princess Haya Biotechnology Center, Jordan University of Science and Technology, Irbid 22110, Jordan.
Abstract
Background and Aim: MicroRNAs (miRNAs) play an important role in various biological functions. According to many studies, miRNA expression is tissue-specific, strongly controlled throughout embryogenesis, and over- or under-expressed in numerous disorders, including cardiovascular pathologies. This study aimed to screen, characterize, and profile many induced biomarkers (miRNAs) in dog serum before and after experimentally inducing a regional myocardial infarction (MI) by occluding the coronary arteries under general anesthesia.
Materials and Methods: A preclinical experimental animal study recruited 12 healthy canine dogs. The selected canine dogs were anesthetized with 1 mg/kg xylazine and 15 mg/kg ketamine before undergoing femoral arterial catheterization under fluoroscopic supervision. Commercial assay kits were used to purify total RNA and miRNA before the occlusion and 2 h after the occlusion according to the manufacturer’s guidelines, and the samples were stored in RNase/DNase-free water at –80°C. Data were analyzed by GraphPad Prism 5.0 software (GraphPad Prism, San Diego, CA) SPSS, and GenEx software (www.multid.se) or (REST V3).
Results: Among 325 transcribed genes, 20 were identified in 2 h. After MI, 14 biomarkers were negative, indicating downregulation, and 6 (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, 3-F10) were positive, indicating upregulation. Polymerase chain reaction assay results showed a normalized fold-change in gene expression in the test sample. Fold values >1 represented a biologically significant change.
Conclusion: Profiling of miRNAs before and after MI in a dog model revealed upregulation of six previously unidentified biomarkers (3-F08, 3-B10, 4-A11, 1-A06, 2-E01, and 3-F10), indicating various miRNA regulatory patterns.
Keywords: dog model, heart infarction, microRNAs, veterinary.
Funder
Jordan University of Science and Technology
Publisher
Veterinary World
Subject
General Veterinary
Reference26 articles.
1. Lou, S., Sun, T., Li, H. and Hu, Z. (2018) Mechanisms of microRNA-mediated gene regulation in unicellular model alga Chlamydomonas reinhardtii. Biotechnol. Biofuels, 11: 244. 2. Kiyohara, K., Sakai, T., Nishiyama, C., Nishiuchi, T., Hayashi, Y., Iwami, T. and Kitamura, T. (2018) Epidemiology of out-of-hospital cardiac arrest due to suffocation focusing on suffocation due to Japanese rice cake: A population-based observational study from the Utstein Osaka project. J. Epidemiol., 28(2): 67–74. 3. Bray, F., Laversanne, M., Weiderpass E. and Soerjomataram, I. (2021) The ever‐increasing importance of cancer as a leading cause of premature death worldwide. Cancer, 127(16): 3029–3030. 4. Li, H., Hastings, M.H., Rhee, J., Trager, L.E., Roh, J.D. and Rosenzweig, A. (2020) Targeting age-related pathways in heart failure. Circ. Res., 126(4): 533–551. 5. Venosa, A. (2020) Senescence in pulmonary fibrosis: Between aging and exposure. Front. Med. (Lausanne), 7: 606462.
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