First study on microscopic and molecular detection of Acanthocheilonema reconditum and Leishmania infantum coinfection in dogs in Southwest Colombia
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Published:2023-01-12
Issue:
Volume:
Page:94-103
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Pérez-Ramírez Ruben Dario1ORCID, Lugo-Vargas Rodrigo1ORCID, Petano-Duque Julieth Michel1ORCID, Cruz-Méndez Juan Sebastian2ORCID, Rondón-Barragán Iang Schroniltgen2ORCID
Affiliation:
1. Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia. 2. Research Group in Immunobiology and Pathogenesis, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Ibagué-Tolima, Colombia.
Abstract
Background and Aim: Canine vector-borne diseases represent an important issue for the welfare and health of animals, but also have great zoonotic potential. These diseases are caused by bacteria, nematodes such as filariae, and other parasites such as Leishmania spp. Given the difficulty in differentiating common microfilariae in dogs by microscopy and serological methods, molecular techniques such as polymerase chain reaction (PCR) and sequencing should be valuable for reaching a reliable diagnosis. This study aimed to use microscopy and PCR to identify the microfilarial species in dogs from Valle del Cauca, Colombia, and a possible association with Leishmania infantum parasites.
Materials and Methods: This study was conducted on 270 dogs from Pradera and Florida municipalities. Microfilariae were detected in dogs by optical microscopy and amplification with 5.8S-ITS2-28S. Species identification was achieved through the amplification of the gene cytochrome oxidase I (COX1).
Results: Microscopic detection of microfilariae was possible in 4.81% (13/270) of the dogs. In addition, by PCR of COX1 and Sanger sequencing of ITS2, Acanthocheilonema reconditum was identified as the circulating microfilarial species in 12 dogs, coinfecting with the species L. infantum (Leishmania donovani complex).
Conclusion: To the best of our knowledge, this is the first report on A. reconditum and L. infantum mixed infection in dogs in Colombia, particularly in the Valle del Cauca.
Funder
Universidad del Tolima
Publisher
Veterinary World
Subject
General Veterinary
Reference68 articles.
1. Colombo, M., Morelli, S., Simonato, G., Di Cesare, A., Veronesi, F., di Regalbono, A.F., Grassi, L., Ilaria, R., Tiscar, P.G., Morganti, G., Hattab, J., Rizzo, V. and Traversa, D. (2021) Exposure to major vector-borne diseases in dogs subjected to different preventative regimens in endemic areas of Italy. Pathogens, 10(5): 507. 2. Radzijevskaja, J., Tamoliūnaitė, D., Sabūnas, V., Aleksandravičienė, A. and Paulauskas, A. (2020) Prevalence and co-infection of mosquito- and tick-borne pathogens in domestic dogs suspected for canine babesiosis in Lithuania. Biologija, 66(2): 94–102. 3. Gizzarelli, M., Foglia-Manzillo, V. and Ciuca, L. (2019) Simultaneous detection of parasitic vector-borne diseases: A robust cross-sectional survey in hunting, stray and sheepdogs in a Mediterranean area. Front. Vet. Sci., 6 : 288. 4. Panarese, R., Iatta, R., Beugnet, F. and Otranto, D. (2022) Incidence of Dirofilaria immitis and Leishmania infantum infections in sheltered dogs from Southern Italy. Transbound. Emerg. Dis., 69(2): 891–894. 5. Hoseini, M., Jalousian, F., Hoseini, S.H. and Sadeghian, S.H. (2020) A cross-sectional study on Dirofilaria immitis and Acanthocheilonema reconditum in sheepdogs in a western region in Iran. Vet. Res. Forum., 11(2): 185–190.
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