The frequency of virulent genes and antimicrobial resistance patterns of diarrheagenic Escherichia coli isolated from stools of children presenting with diarrhea in a tertiary hospital in Abakaliki, Nigeria
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Published:2020
Issue:2
Volume:6
Page:147-152
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ISSN:2455-8931
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Container-title:July-December
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language:en
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Short-container-title:Int J One Health
Author:
David Ebuka Elijah1ORCID, Yameen Muhammad Arfat2, Igwenyi Ikechuku Okorie3, Okafor Arthur Chinedu4ORCID, Obeten Uket Nta1, Obasi Doris Olachi1, Ezeilo Uchenna Regina1ORCID, David Chidinma Nnenne5
Affiliation:
1. Department of Chemistry/Biochemistry, Alex-Ekwueme Federal University Ndufu-Alike, Ikwo, Ebonyi State, Nigeria. 2. Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, Pakistan. 3. Department of Biochemistry, Ebonyi State University, Ebonyi State, Nigeria. 4. Department of Microbiology, Edo University, Iyamho, Edo State, Nigeria. 5. Department of Microbiology, Ebonyi State University, Ebonyi State, Nigeria.
Abstract
Aim: This study was aimed to determine the virulent genes and antibiotic resistance patterns among circulating diarrheagenic Escherichia coli (DEC) pathotypes in a tertiary care health center in east of Nigeria.
Materials and Methods: Diarrheal stool samples were obtained from 80 children under 5 years and E. coli was isolated and identified using standard biochemical and molecular methods. Multiplex polymerase chain reaction (PCR) was used to detect eight virulent genes of DEC. Disk diffusion method was used to determine the antibiotic susceptibility of DEC.
Results: DEC infection was observed in 54 (68%) children among which ial gene for enteroinvasive E. coli (EIEC) (40% [n=22]) was commonly detected followed by eltA/eltB for enterotoxigenic E. coli (ETEC) (30% [n=16]), pCVD for enteroaggregative E. coli (EAEC) (20% [n=11]), and eaeA/bfpA for typical enteropathogenic E. coli (EPEC) (10% [n=5]). The DEC isolates phenotypically exhibited resistance for ampicillin (AMP) (44 [81%]), followed by ciprofloxacin (CIP)/ levofloxacin (LEV) (28 [52%]), cefoxitin (FOX) (11 [20%]), and amoxicillin-clavulanic acid (AMC) (6 [11%]). About 60% isolates of stable toxins-ETEC were resistant to AMC, CIP, and LEV while all the labile toxin-ETEC exhibited resistance to AMP. About 60% (n=6) resistance were seen in EAEC against ampicillin, AMC, FOX, CIP, and LEV. In EIEC, all the isolates (n=22) were resistant to AMP while 50% (n=11) were resistant to both CIP and LEV. All EPEC (n=5) were resistant to AMP, FOX, CIP, and LEV.
Conclusion: High frequency of virulent ial and eltA/eltB genes for EIEC and ETEC, respectively, suggests that they are the primary etiological agents of diarrhea in children among DEC pathotypes. Resistance of DEC to more than two classes of antibiotics indicate possible emergence of multidrug resistance.
Funder
Higher Education Commission, Pakistan
Publisher
Veterinary World
Subject
Infectious Diseases,Public Health, Environmental and Occupational Health,Health Policy,General Veterinary
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