Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed-Reference-Cited by-同舟云学术

Expression profiling of heat shock protein genes in whole blood of Romosinuano cattle breed

Published:2023-03 Issue: Volume: Page:601-606
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Taborda-Charris Juan Camilo¹^ORCID,Rodríguez-Hernández Roy²^ORCID,Herrera-Sánchez María Paula²^ORCID,Uribe-García Heinner Fabian¹^ORCID,Otero-Arroyo Rafael J.³^ORCID,Naranjo-Gomez Juan Sebastian¹^ORCID,Lozano-Villegas Kelly Johanna⁴^ORCID,Rondón-Barragín Iang Schroniltgen⁴^ORCID

Affiliation:

1. Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia.

2. Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.

3. Grupo de Investigación en Reproducción y Mejoramiento Genético Animal, Facultad de Ciencias Agropecuarias, Universidad de Sucre, Sincelejo 700001, Sucre, Colombia; Laboratorio de Reproducción Animal, Corporación de Ciencias Biotecnológicas, Embriotecno, Montería 230029, Córdoba, Colombia.

4. Immunobiology and Pathogenesis Research Group, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Altos the Santa Helena, A.A 546, Ibagué 730006299, Colombia; Poultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, Universidad del Tolima, Santa Helena Highs, Postal Code 730006299, Ibagué-Tolima, Colombia.

Abstract

Background and Aim: Heat shock proteins are highly conserved proteins that work as molecular chaperones expressed in response to thermal stress. This study aimed to determine the expression profile of genes related to the heat stress response in whole blood obtained from the Romosinuano creole breed. Materials and Methods: Real-time polymerase chain reaction was performed to analyze the transcript of hsp90, hsp70, hsp60, and hsf1 in the whole blood of Romosinuano under different temperature-humidity indices (THIs). Results: The expression levels of the hsp70 and hsf1 genes at the high-THI level were higher (p = 0.0011 and p = 0.0003, respectively) than those at the low-THI level. In addition, no differences in the expression levels of the hsp60 and hsP90 genes were detected between the two THIs. Conclusion: The overexpression of hsf1 and hsp70 genes play an important role in protecting cells from damage induced by heat stress. Keywords: climate change, external environment, heat shock proteins, heat stress, mRNA, temperature-humidity index.

Funder

Sistema General de Regalías de Colombia

Publisher

Veterinary World

Subject

General Veterinary

Link

https://www.veterinaryworld.org/Vol.16/March-2023/22.pdf

Reference54 articles.

1. 1. Summer, A., Lora, I., Formaggioni, P. and Gottardo, F. (2019) Impact of heat stress on milk and meat production. Anim. Front., 9(1): 39–46.

2. 2. Henry, R.C., Engström, K., Olin, S., Alexander, P., Arneth, A. and Rounsevell, M.D.A. (2018) Food supply and bioenergy production within the global cropland planetary boundary. PLoS One., 13(3): e0194695.

3. 3. Chao, Q. and Feng, A. (2018) Scientific basis of climate change and its response. Glob. Energy. Interconnect., 1(4): 420–427.

4. 4. Ali, M.Z., Carlile, G. and Giasuddin, M. (2020) Impact of global climate change on livestock health: Bangladesh perspective. Open. Vet. J., 10(2): 178–188.

5. 5. Pires, B.V., Stafuzza, N.B., Lima, S.B.G., Negrão, J.A. and Paz, C.C.P. (2019) Differential expression of heat shock protein genes associated with heat stress in Nelore and Caracu beef cattle. Livest. Sci., 230 : 103839.