Wound Healing Activity Of Extract Of Gratiola Officinalis In Vivo

Abstract

Background and aim — Gratiola officinalis extract has antimicrobial properties, but its wound healing activity has not been described. Material and Methods — The aqueous solution of dry Gratiola officinalis extract was used for treatment of skin wounds with different routes of administration. In the experiment 40 white laboratory male rats with weight of 150±50 grams were subjected to the incised model of flat skin wounds in the interscapular region under the general anesthesia. A morphological study was carried out to assess the effectiveness of the therapy administered. Results — The most effective method of treatment was found to be local application of the extract, which reduced wound healing time by 8 days. Intramuscular and oral administrations reduce healing time by 6 days. Morphological signs of regeneration, which developed under the influence of Gratiola officinalis extract, allowed to estimate the wound healing activity. Clear differentiation and thickness of layers of the newly formed epidermis, a large number of thin-walled vessels and actively proliferating fibroblasts indicate an active regeneration process. Conclusion — This extract may be interesting to specialists in regenerative medicine as a remedy for treatment of wound processes.