Identification of Saltol QTL in the breeding rice samples

Abstract

One of the main problems in most of the world rice-growing regions is soil salinity. Rice is considered a saline sensitive crop, especially at the early stages of development and in the period of maturity. In the Rostov region, rice is grown in the south-eastern parts, where there are currently difficulties with the operation of the existing reclamation facilities. The problem of saline soils for this region is especially urgent, since a significant part of the arable lands has alkali complexes. In order to return the saline lands into exploitation, it is necessary to develop salt tolerant varieties, which, under crop rotation and maintenance, can contribute to soil desalinization. Due to the difficulty of determining salt tolerance only by estimating the phenotype, it is necessary to use molecular markers associated with this trait. Thus, the purpose of the current work was to identify one of the main Saltol QTL in breeding rice samples of the eighth generation (F8) obtained from hybridizing the donor variety NSYC Rc106 with Russian varieties. For that purpose, there have been used such marker-assisted selection methods as DNA isolation, polymerase chain reaction (PCR), electrophoresis on 2% agarose gels, gels’ coloring in ethidium bromide solution, photography in ultraviolet light and evaluation of the obtained electrophoregrams. As a result of the study of 398 breeding rice samples, there have been identified 67 samples with the functional allele of Saltol QTL (6865/3, 6874/2, Don 7343/4, Don 7343/5, Don 7343/6, Don 7343/7, Don 7343/8, Don 7343/9, Don 7343/10, Don 7337/1, Don 7337/3, Don 7337/4, Don 7337/5, Don 7337/6, Don 7337/7, Don 7337/8, etc.). There have been recommended to use these samples in the further breeding process in order to develop new salinity resistant rice varieties.