IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF DRUMSTICK (Moringa olifera) LEAF AGAINST SHRIMP PATHOGENIC BACTERIA VIBRIO PARAHAEMOLYTICUS

Abstract

Aim of the present study was to evaluate the antibacterial effect of ethanolic extract of Moringa olifera leaf against shrimp pathogenic bacteria Vibrio parahaemolyticus. The bacteria were cultured on TCBS agar media and enumerated. The ethanolic M. olifera leaf extract (1 mg/mL) was prepared and its antibacterial activity at different dose (400 µL, 300 µL and 200 µL) was evaluated against V. parahaemolyticus by using disk-diffusion method. The bacteria load of 1.75×104 CFU/mL, 1.63×104 CFU/mL and 1.54×104 CFU/mL was employed for each concentration. It was observed that 400 µL of ethanolic extract of M. olifera showed the highest inhibition zones (11.97 mm, 13 mm and 15.13 mm) but not equal to the inhibition zones (17.23 mm, 18.20 mm and 19.70 mm) by standard antibiotic (oxytetracycline). The present study revealed that ethanolic extract of moringa leaf (1mg/ml) had significant positive effect (P value = 0.003) on different V. parahaemolyticus load  at 5% level of significance though effect of different doses with same concentration (1mg/ml) had not significantly differ from each other. Therefore, the ethanolic extract of M. olifera might be used as alternative way to prevent diseases which are caused by V. parahaemolyticus.