IMMUNOMODULATORY PROPERTIES OF GLYCYRRHIZINIC ACID IN A MODEL OF CYCLOPHOSPHAMIDE-INDUCED TOXICITY AND IMMUNOSUPPRESSION-Reference-Cited by-同舟云学术

IMMUNOMODULATORY PROPERTIES OF GLYCYRRHIZINIC ACID IN A MODEL OF CYCLOPHOSPHAMIDE-INDUCED TOXICITY AND IMMUNOSUPPRESSION

Published:2024-06-25 Issue:2 Volume: Page:50-63
ISSN:2413-4864
Container-title:Acta medica Eurasica
language:
Short-container-title:Acta med. Eurasica

Author:

Danilova Irina M.¹^ORCID,Pavlova Svetlana I.¹^ORCID,Khobrakova Valentina B.²^ORCID

Affiliation:

1. Chuvash State University

2. Institute of General and Experimental Biology, Siberian Branch of the Russian Academy of Sciences

Abstract

Triterpenoid glycyrrhizic acid, which is part of licorice root extract, has a wide range of biological effects. It was previously revealed that due to glycyrrhizic acid the extract increases the survival rate of animals against the background of cyclophosphamide-induced toxicosis. Since cytostatics cause a wide range of side effects, including pronounced suppression of the immune system, further investigation of glycyrrhizic acid's effect on immunity is of interest. The purpose of the study was to evaluate the effect of glycyrrhizic acid on the immune response in vitro and in experimental animals against the background of cyclophosphamide administration. Materials and methods. The study was carried out in vitro on mice splenocyte culture, as well as on models of cyclophosphamide-induced toxicity and immunosuppression using cyclophosphamide at the dose of 450 mg/kg and 250 mg/kg. The survival rate of animals, the morphological presentation of splenocytes, the content of acid phosphatase, the state of cellular and humoral immunity and phagocytic activity were evaluated. Research results. Glycyrrhizic acid did not inhibit proliferation of mitogen-activated lymphocytes. Administration of glycyrrhizic acid was found to improve the morphological presentation of the spleen by virtue of increasing the size of lymphoid follicles and reducing foci of necrosis against the background of cyclophosphamide-induced toxicosis and to reduce the amount of acid phosphatase. Against the background of cyclophosphamide-induced immunosuppression, administration of glycyrrhizic acid increases the index of delayed hypersensitivity reaction, increases the number of antibody-forming cells compared with the control, improves the phagocytic activity. Conclusions. Glycyrrhizic acid improves the indices of the immune response both in vitro and in modeling cyclophosphamide-induced immunosuppression in laboratory animals. Glycyrrhizic acid improves the morphological presentation of the spleen, as well as enhances cellular, humoral immunity, the phagocytic response against the background of cyclophosphamide administration.

Publisher

I.N. Ulianov Chuvash State University

Link

https://acta-medica-eurasica.ru/wp-content/uploads/2015/07/AME_2024_2_50-63.pdf

Reference28 articles.

1. Murav’ev I.A., Ponomarev V.D. Sovremennoe sostoyanie issledovanii glitsirrizinovoi i glitsirretovoi kislot i ikh proizvodnykh [Current state of researches on glycyrrhizic and glycyrrhetic acids and their derivatives]. In: Voprosy izucheniya i ispol’zovaniya solodki v SSSR: sbornik nauchnykh trudov [Issues of studying and using licorice in the USSR: Collected paper]. Moscow, Nauka Publ., 1966, pp. 118–

2. Pavlova S.I., Andreeva N.A., Khobrakova V.B. Tsitotoksichnost’ izolikviritigenina po otnosheniyu k kletkam opukholevogo i neopukholevogo proiskhozhdeniya [Isoliquiritigenin cytotoxicity against to tumor and non-tumor cells]. Voprosy obespecheniya kachestva lekarstvennykh sredstv, 2019, no. 1(23), 53–62.

3. Pavlova S.I. Immunosupressivnye i protivoopukholevye farmakodinamicheskie effekty flavonoidov kornei solodki: dis. … d-ra med. nauk. [Immunosuppressive and antitumor pharmacodynamic effects of licorice root flavonoids. Doct. Diss.]. Moscow, 2012, 236 p.

4. Pavlova S.I. Ispol’zovanie ekstrakta kornya solodki dlya povysheniya effektivnosti terapii zlokachestvennykh novoobrazovanii (eksperimental’noe issledovanie: … cand. med. nauk. [Improvement the effectiveness of cancer therapy by use of licorice root extract (experimental study). Cand. Diss.]. Staraya Kupavna, 2005, 106 p.

5. Rukovodstvo po provedeniyu doklinicheskikh issledovanii lekarstvennykh sredstv. Chast’ pervaya [Guidelines for conducting preclinical studies of drugs. Part one]. Moscow, Grif i K Publ., 2012, 944 p.