Molecular phylogeny and morphology reveal two new graminicolous species, Curvularia aurantia sp. nov. and C. vidyodayana sp. nov. with new records of Curvularia spp. from Sri Lanka

Abstract

Despite being a small island, Sri Lanka is rich in fungal diversity. Most of the fungi from Sri Lanka have been identified as pathogens of vegetables, fruits, and plantation crops to date. The pleosporalean genus Curvularia (Dothideomycetes) includes phytopathogenic, saprobic, endophytic, and human/animal opportunistic pathogenic fungal species. The majority of the plant-associated Curvularia species are known from poaceous hosts. During the current study, 22 geographical locations of the country were explored and collections were made from 10 different poaceous hosts. Morphology and molecular phylogeny based on three loci, including nuclear internal transcribed spacers 1 and 2 with 5.8S nrDNA (ITS), glyceraldehyde-3phosphate dehydrogenase (gapdh), and translation elongation factor 1-α (tef1) supported the description of two new species of fungi described herein as C. aurantia sp. nov. and C. vidyodayana sp. nov. Moreover, novel host-fungal association records for C. chiangmaiensis, C. falsilunata, C. lonarensis, C. plantarum, and C. pseudobrachyspora are updated herein. In addition, five species within the genus Curvularia, viz., C. asiatica, C. geniculata, C. lunata, C. muehlenbeckiae, and C. verruculosa represent new records of fungi from Sri Lanka.