Cellular Regulation by Hydrogen Peroxide

Abstract

ABSTRACT. Substantial evidence suggests that the transient production of H2O2 is an important signaling event triggered by the activation of various cell surface receptors. Understanding the intracellular messenger function of H2O2 calls for studies of how receptor occupation elicits the production of H2O2, what kinds of molecules are targeted by the produced H2O2, and how H2O2 is eliminated after the completion of its mission. Recent studies suggest that growth factor–induced H2O2 production requires the activation of PtdIns 3-kinase. The essential role of PtdIns 3-kinase is likely to provide PI(3,4,5)P3 that recruits and activates a guanine nucleotide exchange factor of Rac, which is required for the activation of NADPH oxidase. The targets of H2O2 action include proteins that contain a reactive Cys residue. Thus, H2O2 produced in response to growth factor causes inactivation of protein tyrosine phosphatases in various cells by oxidizing specifically the catalytic Cys. These results, together with other observations, indicate that the activation of a receptor tyrosine kinase per se by binding of the corresponding growth factor might not be sufficient to increase the steady-state level of protein tyrosine phosphorylation in cells. Rather, the concurrent inhibition of protein tyrosine phosphatases by H2O2 might also be required. Peroxiredoxins, members of a newly discovered family of peroxidases, efficiently reduced the intracellular level of H2O2 produced in the cells stimulated with various cell surface ligands. Furthermore, the activity of peroxiredoxin enzymes seems to be regulated via protein phosphorylation as in the case of many other intracellular messenger metabolizing enzymes. E-mail: sgrhee@nih.gov